1.For patients with regular menstruation, please take blood test for endocrine six, TORCH, ASAb, EMAb, TSH and TPO-Ab at 8-11 am on the 1st to 3rd day of menstruation. 2.Patients with irregular menstrual cycle and amenorrhea: check urine HCG to exclude pregnancy, and check endocrine six, TORCH, ASAb, EMAb, TSH and TPO-Ab if both ovaries are in the basal state. 3.Coitus is prohibited for 3-7 days after menstruation: check leukorrhea routine, urine pregnancy test and ECG in the morning if there is no abnormality, and do hysterosalpingography in the afternoon. 4, 21-23 days after menstruation, or 1 week after ovulation, 8-11 am blood test E2, P to understand your luteal function. 5.If your menstrual cycle is regular for 28-30 days, please have a vaginal ultrasound on the 10th-12th day of menstruation to monitor the follicle development. 6.Male forbids coitus for 2-7 days to check semen routine.