Fatigue test: Both eyes keep looking upward, and the eyelids are seen to come down gradually, or the eye fissure becomes significantly smaller after blinking hard 30 times. Neostigmine test: Neostigmine 1mg/atropine 0.5mg, observe 20min-1h, 30% improvement is positive. Immunological indexes: 70%-80% of patients with systemic type are positive for AchR antibodies, the positive rate of ocular muscle type is low, MuSK antibodies can be detected in about 40% of AchR-negative patients, and Titin and Ryanodine (rhabdomyosarcoma antibodies) antibodies can be measured with thymoma Those who often combine with hyperthyroidism can have increased T3 and T4 and decreased TSH, or combine with other autoimmune diseases need to be detected Anti-nuclear antibodies, rheumatic series, etc. EMG: Repeated electrical stimulation at low frequency (2-5Hz) with more than 10% attenuation is positive, and bromipyridostigmine should be stopped for 12-18 hours before the test. Thymus CT enhancement: Thymic hyperplasia or thymoma is associated with severe myasthenia gravis. Patients with thymoma and thymic hyperplasia over 18 years of age should be considered for surgery, some patients can be relieved after surgery, some patients still need medication.