In vitro fertilization and embryo transfer (IVF-ET) is often called “in vitro fertilization” in our country. In fact, in vitro fertilization is a special technique in which both eggs and sperm are taken outside the body and allowed to complete the fertilization process in an artificially controlled environment outside the body, and then the early embryos are transferred to the woman’s uterus where they are conceived as a child. Babies produced using in vitro fertilization techniques are called IVF, and these children also grow up in the mother’s womb. It can be said that “IVF technology” is equivalent to “in vitro fertilization”. 1. Physical factors of the operator. Physical factors can be subdivided into four aspects: age, chromosomes, intrauterine environment and infertility symptoms (1) Age. Age is an important factor affecting the success rate of IVF, and is also a reflection of a normal and vigorous female reproductive system. As we age, the number of eggs decreases, the quality decreases, the fertilization rate decreases, the pregnancy rate decreases significantly, and the miscarriage rate increases. Studies say that the pregnancy rate for couples of childbearing age is only 20%. After women reach the age of 35, all body functions decline, with the reproductive system being particularly prominent. Domestic and international literature reports that the success rate of IVF surgery is highest when the age is 25-34 years old, and the success rate decreases significantly after the age of 35 years old, and the success rate is around 20% when the age is older than 40 years old, and the live birth rate decreases significantly, and it also has a greater impact on eugenics. When over 45 years old generally cannot do IVF treatment with their own eggs because the success rate is extremely low and the chromosomes of the embryos are prone to abnormalities. the miscarriage rate increases greatly in IVF treatment in the older age groups, and the preterm births and stillbirths are also higher than in younger pregnant women. (2) Chromosomes. In some couples with primary infertility or a history of adverse pregnancy, if the couple has normal chromosomal variants, although they can conceive through IVF technology, the failure rate and miscarriage rate are higher. (3) Intrauterine environment. Such as endometrial polyps, endometritis, previous surgery or inflammation (tuberculosis is the most common) resulting in endometrial damage can affect embryo implantation, thus leading to IVF procedure failure. (4) Patients with infertility symptoms Older primary infertility, patients with severe endometriosis and polycystic ovary syndrome with uterine malformation, those who do not get sperm from the testes of patients with severe oligospermia or azoospermia, or those who have a large number of malformed sperm, and those with malformed eggs or abnormal development, etc., also have a high failure rate in IVF treatment. 2. Excellent embryo rate When there are problems with sperm and eggs, no fertilization or fertilization without egg cleavage can’t complete embryo transfer, excellent embryo is an important condition for success. However, excellent embryos also need good uterine environment, good endocrine conditions, and doctors’ superior embryo transfer techniques. Couples of normal fertile age who have intercourse at the time of ovulation for 1 month have only about 20% chance of pregnancy. So we hope to have a good attitude during the treatment, which is also an important factor for success. Therefore, the excellent embryo rate factor is closely related to the physical factors of the operator. Based on the current medical technology, it can be said that the physical condition of the couple undergoing artificial insemination determines the success rate of excellent embryos. 3. Psychological state factor Many data show that the psychological and mental state of the patient is an important factor affecting the success or failure of IVF treatment. Experts believe that women with heavy psychological stress will have their endocrine secretion affected and their blood vessels will be in a state of contraction for a long time, which affects the local blood flow to the uterus and ovaries, and the tension in the nervous system will cause abnormalities in the release of some neuromediators, resulting in disorders in the contraction of the muscles of the uterus and fallopian tubes, causing the embryo not to be able to lay and leading to treatment failure. Therefore, it is very important to relieve the psychological stress, communicate between the doctor and the patient, and have mutual understanding and encouragement between the couple.