For patients who are not familiar with assisted reproductive technology may not understand the indications and specific options of IVF, such as not knowing whether they are doing first generation IVF or second generation IVF, and some patients even think that the higher the level of IVF technology, the higher the success rate, which is actually not true. The main difference between first generation, second generation or third generation IVF is that the patients who are adapted to it are different. Today, we will talk to you about these three technologies and their indications and contraindications. First generation IVF (IVF): This is what we call conventional IVF. It is a technique in which the eggs and sperm of a couple with infertility are removed from the body, the sperm is washed so that the eggs are fertilized in the in vitro system and developed into embryos, and then the embryos are transferred into the uterine cavity to achieve pregnancy. The fertilization of eggs in this process is close to the fertilization of eggs in a natural pregnancy. Indications 1.Obstruction of egg transport caused by various factors in the female partner, such as bilateral tubal obstruction, tubal agenesis, severe pelvic adhesions and other tubal loss; 2.Ovulation disorders: refractory ovulation disorders that have not obtained pregnancy after repeated conventional treatments, such as repeated ovulation induction or combined with intrauterine insemination treatment; 3.Endometriosis: endometriosis that has not obtained pregnancy after conventional drug or surgical treatment; 4.Male endometriosis Male factor infertility: male infertility with few or weak teratozoospermia or a combination of factors, where pregnancy has not been obtained after intrauterine insemination treatment, or where the severity of the male factor is not suitable for intrauterine insemination; 5. Immune infertility and unexplained infertility: where pregnancy has not been obtained after repeated intrauterine insemination treatment or other conventional treatments. In vitro fertilization of the second generation (ICSI): intracytoplasmic single sperm injection is the injection of a single sperm into the oocyte plasma by microinjection, thus is the passive combination of sperm and oocyte fertilization, the formation of a fertilized egg and embryo transfer, to achieve pregnancy. Indications 1. Severe oligo-teratospermia; 2. Irreversible obstructive azoospermia; 3. Spermatogenic dysfunction (excluding those caused by genetic defects); 4. Immunological infertility; 5. Failed fertilization by conventional IVF or very low fertilization rate; 6. Sperm acrosome abnormalities and other conditions. Third generation IVF (PGD): Pre-implantation embryo genetic diagnosis means taking some cells from in vitro fertilized embryos for genetic testing to exclude embryos with disease-causing genes before transfer. Indications 1. Patients with abnormal chromosome number or structure; 2. One of the spouses is a carrier of a sex-linked heritage disease (e.g. hemophilia, pseudohypertrophic muscular dystrophy); 3. Patients or carriers of monogenic diseases for which genetic diagnosis is available, etc. Contraindications to assisted reproductive technology: 1. serious mental illness, acute infection of the genitourinary system and sexually transmitted diseases in either sex; 2. genetic diseases that are not suitable for childbirth and cannot be diagnosed prenatally or by pre-implantation genetics as stipulated in the Maternal and Child Health Law; 3. serious drug addiction in either spouse; 4. 5. Couples in which the uterus of the female partner does not have the function of pregnancy or has serious physical diseases that cannot bear pregnancy or has other contraindications to IVF-ET. After the introduction of this knowledge I believe you have a certain understanding of IVF treatment, in the selection of the program should also try to listen to the advice of the attending doctor to choose the right program for your treatment.