Gamete transportation disorder (double tubal obstruction, tubal adhesion treatment within 1 year is still infertile) 2, ovulation disorder patients (amenorrhea, menstrual disorders, scanty menstruation, follicle does not rupture), ovulation treatment and 3 cycles of artificial insemination is still infertile. 3.Men with moderate or severe oligozoospermia, or men with azoospermia who need to obtain sperm by testicular or epididymal puncture; 4.Endometriosis with infertility; 5.Unidentified infertility, failure to conceive through general ovulation therapy and 3 cycles of artificial insemination; 5.Understandable infertility; 6.Understandable infertility, failure to conceive through general ovulation therapy and 3 cycles of artificial insemination; 7.Unidentifiable infertility.