1, tubal imaging is within a week of menstruation, to vaginal cleanliness of one degree or better, do not have sex. 2, the line of sex hormone measurement, usually for the second to fifth day of menstruation, fasting blood draw, and it is best to come to the hospital earlier, rest 30 minutes before drawing blood. 3.Monitoring ovulation. Generally, women ovulate about 2 weeks before the next period, and accompanied by elevated progesterone, resulting in elevated basal body temperature, so it is recommended that the patient perform basal body temperature measurement, plus progesterone, combined with ultrasound, usually at least once before ovulation to clarify the size of the follicle, and once after ovulation to determine whether there is ovulation. 4, male semen examination: generally in 2~5 days without intercourse, it is recommended to check about 3 times, once does not explain the problem. 5, endometrial thickness: usually in the middle of menstruation, i.e. ovulation endometrium, if there is any abnormality, it is recommended to check again within 2~3 days after the next menstruation, and there is abnormality that can be checked by hysteroscopy. 6.For patients with abnormally high prolactin level? It is recommended to perform magnetic resonance examination more accurately.