”In vitro fertilization and embryo transfer (IVF-ET), the medical name for IVF, is a method of artificially assisted conception that aims to help infertile couples achieve a healthy child. “IVF-ET also includes a number of derivative techniques: intracytoplasmic sperm injection (ICSI), embryo freezing, embryo assisted hatching (AH), in vitro maturation of immature oocytes (IVM), pre-implantation genetic diagnosis (PGD), pre-implantation genetic screening (PGS), egg donation, etc. Couples who come to the fertility center should first complete the necessary examinations and diagnoses and meet the indications for IVF treatment, such as tubal blockage, ovulation disorder, immune infertility, endometriosis, male oligospermia, obstructive azoospermia, unexplained infertility, etc., and complete the “identity card”, “marriage certificate, birth certificate” and “birth certificate”. After the verification of the three documents: ID card, marriage certificate, birth certificate, etc., the patient can enter the IVF treatment procedure. In vitro fertilization-embryo transfer (IVF-ET) is the process of fertilization of sperm and egg in an in vitro laboratory environment, followed by the transfer of the formed embryo back to the woman’s uterus. In IVF, the patient first receives medication to develop and mature multiple follicles. When the eggs are about to be expelled, the doctor will use an egg retrieval needle under ultrasound guidance to puncture the mature follicles through the vagina and aspirate the eggs inside. Analgesics or general anesthesia can help reduce or even eliminate pain during the egg retrieval procedure. In vitro fertilization can be performed in two ways: IVF and ICSI. usually, the embryologist will place the eggs with the preferentially selected sperm and monitor the eggs for fertilization. However, if the male partner’s sperm is of poor quality, or if fertilization in previous IVF cycles was too poor, the embryologist will select one of the most viable and morphologically optimal sperm and inject it into the egg manually to help fertilize the egg, a technique called “intracytoplasmic sperm injection” (ICSI). Assisted hatching is the use of physical or chemical methods to artificially create a defect or cleft in the zona pellucida of the embryo to facilitate the “breaking” of the embryo from the zona pellucida or the dissolution of the zona pellucida to facilitate embryo hatching and implantation, increasing the likelihood of implantation. Indications for assisted hatching: 1. Elevated basal level of FSH (FSH >15miu/ml on the 3rd day of menstruation). 2. History of failed IVF treatment, when obvious factors such as endometrium and embryo quality are excluded. 3.When the female partner is older than 38 years old. 4.Zona pellucida abnormalities such as irregular morphology, thick zona pellucida, deep coloring, etc. 5.Frozen embryos. A few days after egg retrieval or after thawing of frozen embryos, an embryo will be placed back into the woman’s uterus through the vagina, a process called “embryo transfer (ET)”. Transferring the right number of embryos is effective in reducing the incidence of multiple pregnancies. Good quality embryos that are not transferred can be frozen (embryo freezing) for future use.