1.Sperm vitality and morphological examination for men; 2.Sex hormone six tests for women; 3.Female eugenics four tests; 4.Full immunological examination; 5.Thyroid function examination; 6.Bacterial vaginitis screening by white belt routine; 7.B ultrasound follicle monitoring; Deng Jie, Department of Obstetrics and Gynecology, Xiangyang First People’s Hospital; 8.ABO hemolysis and Rh blood group antigen system examination; 9.Chromosome examination for both men and women; 10.Hysteroscopy if necessary