The correct term for the first generation of IVF is in vitro fertilization and embryo transfer, and the term “in vitro fertilization” is just a common name. In fact, the name “IVF” was given to the early stages of assisted reproductive technology because the embryos were cultured in test tubes. Nowadays, eggs and sperm are cultivated separately in specialized vessels, and then the eggs are fertilized and split into early embryos with 4 or 8 cells, which are then transferred into the human uterus for further growth and development until delivery. In clinical practice, the first generation of IVF is the most used, and hundreds of thousands of IVF babies have been born worldwide. The first IVF baby was born in 1978 as Louise Brown. The first IVF baby was born in 1978, named Louise Brown, who has now given birth to the next generation and is a happy mother. The first generation of IVF is suitable for patients with infertility caused by blocked fallopian tubes and endometriosis. The correct term for the second generation of IVF is intracytoplasmic single sperm injection. The egg is held in place by a special fixation needle and a sperm is then aspirated by a thin puncture needle, which penetrates the permeable band outside the egg cell and the oocyte membrane; when the puncture needle enters the cytoplasm, the sperm is injected into the cytoplasm of the egg and fertilized. The embryo is then transferred to the human uterus where it continues to grow and develop until delivery. The second generation of IVF was first tested in Belgium in 1992, mainly for men with little or no sperm, but with a small amount of sperm in the seminiferous tubules. Through puncture, 1D2 sperm can be aspirated, which may solve the male infertility problem. The third generation of IVF actually focuses on genetic diagnosis before embryo implantation. As with the first and second generations of IVF, embryos are obtained through in vitro fertilization. When the embryo has developed to a 4D8 cell embryo, one or two cells (commonly referred to as oocytes) are removed under a microscope for genetic testing, and their integrity is maintained. If it is confirmed that no genetic abnormality has occurred in the embryo, it is then transferred to the patient’s uterus to continue its growth and development. This method has been successful since 1989. With 1/5D1/4 of the population suffering from hereditary diseases, each carrying an average of 5D6 recessive genes, it would greatly improve the quality of births if the presence of hereditary diseases could be identified before embryo transfer. Of course, this is easier said than done. Because there are more than 4,000 genetic diseases in the world, and the current prenatal diagnosis of the third generation of IVF is only about 30, it is not possible to guarantee the quality of the child born later. The third generation of IVF has played a role in solving female infertility and male infertility, as well as preventing genetic diseases. However, there are two sides to every story, and IVF is not immune to them. Until 1998, there were only about 100 cases of third-generation IVF in the world. This is an indication of the technical difficulty of the procedure and the range of problems that remain to be solved. The removal of 1D2 dividing spheres from early embryos may cause damage to the embryo or later cause a miscarriage. It is difficult to conclude whether there are any future effects on the born babies, and more cases and longer time are needed to verify this. Since 2009, we have seen 19 couples for genetic diagnosis and 21 cycles, and 7 healthy babies were born in the third generation of IVF, including 3 males and 4 females; the patients who got happy and healthy babies through our technology include patients with Crohn’s syndrome, Fernando and (13.14) balanced ectopic patients. From the above, we can see that each generation of IVF has its own advantages and disadvantages for the mother and the baby, which should be properly understood.