Female examination: 1. Physical examination: check the development of genitalia and secondary sex characteristics, height and weight, growth and development, hairiness, breast overflow, etc. If necessary, chest X-ray to exclude tuberculosis, MRI to exclude pituitary lesions, etc. 2. Ultrasound imaging: Ultrasound examination is a common means to diagnose infertility, which has the advantages of non-invasive, convenient, high detection rate and accuracy, and can be recorded by camera for comparison, etc. B-mode ultrasound examination can detect lesions of uterus and ovaries. B-mode ultrasonography can show the number of ovarian sinus follicles to determine the reserve function of the ovaries. 3. Determination of ovulation and endocrine function: commonly used methods: basal body temperature measurement, cervical mucus scoring, serum endocrine hormone testing and ultrasound monitoring of follicle development and ovulation. Hormone testing often includes the examination of serum FSH, LH, E2, P, TSH and PRL. The measurement of hormones is most important with the detection of serum basal endocrine levels on days 2-5 of the menstrual cycle, which can reflect the basal state of the ovaries and their reserve capacity or certain pathological states. Mid-luteal serum E and P levels reflect ovarian luteal function. Elevated basal FSH levels indicate decreased ovarian reserve capacity. Elevated serum basal LH/FSH ≥2 and T may assist in the diagnosis of polycystic ovary syndrome. If necessary, thyroid and adrenocortical function and other endocrine functions should be measured to rule out abnormal ovarian function due to systemic endocrine abnormalities. 4.Tubal patency test: the main tests are: uterine tubal lavage, uterine tubal iodography, and laparoscopic direct view tubal lavage (Melanin). Tubal lavage is a simple and inexpensive test, but the accuracy rate is not high. Hysterosalpingography with iodine shows the uterus and fallopian tubes. Performing tubal lavage under direct laparoscopic view is a more objective and accurate method. 5. Examination of the cervix and uterus: In addition to routine gynecological examination, cytology, bacteriology and pathogen examination of vaginal and cervical secretions, cervical mucus score and post-coital test (PTC) can be used. If necessary, hysteroscopy or laparoscopy is performed. 6. Reproductive immunological examination: including sperm antigen, anti-sperm antibody, anti-endometrial antibody, etc. 7, genetic examination: chromosomes, etc. Male examination: 1. Physical examination: genitalia with or without abnormalities and deformities. Penis with or without hypospadias and severe penile curvature, note the presence of penile sclerosis. Bilateral testicular volume, texture and presence of nodules; whether there are abnormalities in the vas deferens. The presence or absence of varicocele; rectal examination of the prostate gland for enlargement and smoothness. 2.Imaging examination: B-type ultrasound, CT, MRI examination. 3.Conventional semen analysis examination and functional examination of spermatozoa. 4.Immunological examination: anti-sperm antibody (AsAb) detection machine seminal plasma immunosuppressive substance (SPIM) determination, etc. 5.Microbiological examination: Ureaplasma urealyticum (UU) infection can cause extensive lesions in seminiferous tubules, resulting in decreased spermatogenic function. 6, genetic examination: chromosomal abnormalities, etc. 7.Endocrine examination: including the measurement of several hormones such as LH, FSH, PRL, T and E, reflecting the function of hypothalamus-pituitary-gonadal axis.