The first generation of “IVF” involves the wife being given ovulation stimulants to mature multiple eggs in her ovaries, which are punctured from her ovaries and placed in a “test tube” under the guidance of a vaginal ultrasound. The husband’s semen is processed and placed in the same “test tube”. The sperm and egg unite (fertilization) in the “test tube”, forming a fertilized egg that divides and develops into a small embryo in the “test tube” (usually the small embryo lives in the “test tube” for 48-120 hours). The embryos are then divided and developed into small embryos in the “test tube” (usually the embryos live in the “test tube” for 48-120 hours). The small embryos are returned to the uterine cavity through a catheter at the right time, and are given medication to preserve the pregnancy. After a certain period of time, the pregnancy is declared a success if it is confirmed by a blood test or ultrasound. Tubal obstruction, endometriosis, oligo/weak spermatozoa, immune infertility, ovulation disorders and unexplained infertility can all be used to assist pregnancy. The second generation of IVF (intracytoplasmic single sperm microinjection) When the husband has too few sperm, weak sperm, too many abnormal sperm, inactive sperm, or no sperm found in the semen, the best autologous or heterologous sperm is injected directly into the egg cell under a microscope to fertilize the egg and divide it into a small embryo and then send it back to the body. Third Generation IVF (Pre-implantation Genetic Diagnosis) Before the embryos are transferred into the uterine cavity, a biopsy of the embryos is taken for chromosome examination, and embryos with genetic abnormalities are eliminated, thus preventing the birth of babies with genetic diseases. Embryo freezing Multiple embryos can be formed after a single superovulation and in vitro fertilization, and the remaining embryos after this transfer can be frozen and stored for another transfer. In vitro fertilization of eggs provided by another woman and implantation into the uterine cavity of an infertile woman. Ovarian dysfunction, repeated failure of in vitro fertilization, poor follicular development or empty follicles, carriers of hereditary diseases, repeated miscarriages due to poor egg quality are eligible for egg donation.