Cytomegalovirus infection is a sexually transmitted disease caused by cytomegalovirus (cmv). Cytomegalovirus is a DNA virus. The characteristic lesions are enlarged infected cells with eosinophilic and basophilic inclusions in the nucleus and cytoplasm, respectively.CMV infection is distributed worldwide and humans are the only host of CMV. The rate of infection varies by country and economic status. There is a close relationship between CMV infection and immune function in adults. What are the screening methods for cytomegalovirus infection? 1, detection in various secretions In recent years, the application of immunoblotting and molecular hybridization techniques directly from the urine, CMV antigen and DNA is both rapid and sensitive, accurate method. 2, detection of intracellular antigen Whether it is the first infection or recurrent infection, when the viraemia, dextran solution can be used to extract peripheral blood single nuclear cells, made into smears, plus CMV monoclonal antibody, using immunoenzymatic or fluorescent staining, detection of intracellular antigen. 3.Electron microscopy Since CMV has a typical herpes virus structure, the virus particles in urine specimens or infected tissues can be detected directly by electron microscopy, and the specificity of this virus particle can also be detected again by specific fluorescent antibodies. 4, serological examination The application of serological techniques such as immunofluorescence, complement binding, enzyme-linked immunosorbent assay and latex agglutination can detect CMV-specific IgG and IgM, which are suitable for early infection and epidemiological investigation. A positive single serum IgG indicates a previous infection, while a 4-fold increase in the potency of a double serum indicates a recent infection, but sometimes it takes several weeks for antibodies to increase, so a recovery specimen should be taken 2-8 weeks after the acute phase. A single positive serum IgM is diagnostic. Since IgM cannot pass through the placenta, a positive newborn cord blood can diagnose congenital infection. 5, pathogenic examination Cell culture to isolate the virus: urine, pharyngeal washings, blood cells and other specimens taken should be placed in the 4 ℃ refrigerator, the best immediate examination, commonly used is the human embryonic fibroblast or foreskin epithelial cell culture, generally in 1-2 weeks can be seen in the cell rounding, agglomeration and other lesions.