Infertility is defined as cohabitation after marriage, having normal sexual life, without contraception for more than one year and failing to conceive. Every married couple carries a beautiful dream of conceiving a child of their own, which is indispensable for every healthy and ordinary family, but infertility is a big blow to the family. The lack of knowledge about infertility has led to many people not paying attention to it in the initial stages, which ultimately leads to infertility, which is a problem that is currently plaguing some families. Infertility is a complex disease caused by a variety of reasons, before the treatment needs to carry out a series of relevant examinations, so that the cause of the disease can be as clear as possible, so as to treat the symptoms of the disease, so here we introduce the infertility of couples need to carry out before the treatment of the examination program, which has a very important significance for a family. (1) Gynecological examination Gynecological examination is the most basic examination carried out by obstetricians and gynecologists on every patient, mainly checking the vagina, cervix and uterus, fallopian tubes, ovaries and paramedian tissues and pelvic cavity lining, and its main function is to make early diagnosis, prevention and early treatment of some gynecological diseases. Gynecological examination has its special characteristics, requiring the patient to take off one trouser leg, including underwear, and take a special position, and then with the help of some simple instruments to help the doctor to understand some of the diseases of women’s sexual reproductive organs. In our country, most women are very resistant to gynecological examination and do not have the consciousness and habit of going to the hospital for regular examination. In fact, gynecological examination is not as terrible as people think, and it protects women’s health to a great extent. (2) Measurement of Basal Body Temperature Basal Body Temperature (BBT), also known as resting body temperature, is the body temperature measured after 6 to 8 hours of sleep, such as waking up from a deep sleep in the morning, when the body temperature has not yet been affected by exercise, diet, or emotional changes, and it is usually the body’s lowest body temperature during the day and night. Basal body temperature is a commonly used, non-invasive method of monitoring the body’s temperature, and requires more than 6 hours of adequate sleep before waking up and doing any activity. Normally, the ovaries of women of childbearing age produce estrogen during the first half of menstruation, and S-body after ovulation. After ovulation, the corpus luteum is formed, and the secretion of progesterone causes the body temperature to rise by about 0.6 degrees Celsius, resulting in a two-phase change in body temperature. The high temperature period will be maintained until the S body atrophy, menstruation until. Therefore, with ovulation body temperature will be slightly increased, on the contrary, anovulatory menstruation lack of hormone action, basal body temperature has no regular cycle changes. Also, the length of the S body period can be used to assess the function of the ovaries. In general, the pyrexia should be more than 12 days to be considered normal ovarian function, and if the pyrexia is under 11 days, there is a possibility of S-body insufficiency, which can assist in the diagnosis of the cause of menstrual disorders. The cause of infertility can be examined through the measurement of basal body temperature: the basal body temperature is measured to understand her ovarian function, the presence of ovulation and luteal function. Determination of ovulation by basal body temperature can guide contraception and fertilization. Basal body temperature can also be measured to assist in the diagnosis of pregnancy. If a high basal body temperature persists for more than two weeks, you should consider going to the hospital for a checkup, because it is very likely that you are pregnant. (3) Cervical Mucus Examination and Scoring Cervical mucus is a hydrogel containing glycoproteins, plasma proteins, sodium chloride and water. The cervical mucus during ovulation is thin and voluminous. The glycoproteins in the cervical mucus are arranged in a mesh, which becomes larger under the influence of oestrogen near the time of ovulation to make it suitable for sperm to pass through. Under normal circumstances, on the 8th to 10th day of the menstrual cycle, crystals can be seen in the mucus smear, and the level of estrogen in the body reaches a peak during ovulation, and typical amniotic crystals appear in the smear. After ovulation, the crystals gradually decrease, and by day 22 the crystals no longer appear. On the 22nd day of the menstrual cycle, the crystals turn into ellipsoids. It is often used to predict ovulation and guide conception, and is more effective when used in conjunction with basal body temperature and vaginal exfoliative cell examination to predict ovulation and select the date of conception. If the smear is all ellipsoids without amniotic crystals, it suggests pregnancy and can diagnose early pregnancy and estimate the prognosis of early pregnancy. Periodic changes in cervical mucus indicate that the ovaries are functioning well and the cause of the lesion is in the uterus, which can assist in identifying the type of amenorrhea. (4) Vaginal cytology The maturity of vaginal squamous epithelial cells is directly proportional to the level of estrogen in the body. The higher the level of estrogen, the more mature the differentiation of vaginal epithelial cells. Therefore, observing the proportion of cells in each layer of vaginal squamous epithelial cells can reflect the level of estrogen in the body, observe the secretion of female hormones, and understand the level of estrogen and progesterone as well as the function of ovaries. (5) Hysterosalpingography Hysterosalpingography (HSG) was adopted in the 1920s. It involves injecting a contrast agent into the uterine cavity through the cervical canal of the uterus, which creates an obvious artificial contrast with the surrounding tissues under X-ray film and makes the lumen of the tubes visible, so as to understand the condition of the uterus and the tubal lumen. Depending on the contrast agent, there are two types: iodized oil and iodized water. Iodized oil has high density, clear visualization, low irritation and does not cause abdominal pain, so it is most commonly used. Infertile patients need to have a hysterosalpingogram to understand the morphology of the uterus, the size of the uterine cavity, bilateral tubal patency and pelvic conditions. This test is safer, does not require anesthesia, causes less damage, is not obviously painful and has a certain therapeutic effect, so it is more acceptable to patients. Suitable for hysterosalpingography infertility patients: ① primary or secondary infertility patients, suspected tubal obstruction. ①Patients with primary or secondary infertility, suspected of tubal obstruction. ②History of abdominal surgery. ③ Observe the shape of uterus to determine whether there are uterine malformations and their types, uterine adhesions, submucosal fibroids, endometrial polyps and foreign bodies. ④ For those who have had several spontaneous abortions in the middle pregnancy and are suspected to have incomplete atresia of the endocervix, observe the endocervix for relaxation in non-pregnancy. Preparation before imaging: ① Choose the time of hysterosalpingography from 3 days after menstruation to before ovulation, i.e., the 7th±4th day of the menstrual cycle. If the time is too early, the endometrium may still have trauma, the contrast medium may enter the uterus from the endometrial trauma peripheral blood vessels, resulting in pulmonary embolism, or the uterine cavity is still residual endometrial debris into the pelvis, artificially causing endometriosis. ② No acute or subacute pelvic inflammatory disease, body temperature below 37.5 ℃. ③ The examination of leukorrhea suspension shows that there is no trichomonas or mycobacterial infection in the vagina. ④ Avoid sexual intercourse and deep water pelvic bathing for 3 days before and 2 weeks after the imaging to prevent infection. Infertile patients who are not suitable for hysterosalpingography: ① Iodine allergy. ② patients in pregnancy, menstruation. ③ Within 6 weeks after postpartum, abortion and curettage. ④ Acute and subacute inflammation of internal and external genitals. ⑤ Those with severe systemic diseases who cannot tolerate the operation. ⑥ Endometrial diagnostic scraping: Diagnostic scraping due to infertility should be chosen before or within 12 hours of menstruation in order to determine whether there is ovulation or not. Those suspected of having endometrial tuberculosis should also undergo endometrial scraping and send it for pathologic examination. In cases of prolonged and heavy bleeding due to post-abortion uterine residue or incomplete shedding of the endometrium, diagnostic scraping not only plays a diagnostic role, but also a therapeutic role.