Sleeping, sleeping, sleeping …… Suddenly, awakened by a signal (gonadotropins), it turned out that the mother-to-be came to the Institute of Reproductive Medicine, and she was doing the preliminary ovulation stimulation work for IVF, and I and a few other siblings became the target of this awakening (follicle recruitment), and we grew and experienced the We grew and experienced the pressure of survival of the fittest (during follicular growth, due to the number and sensitivity of gonadotropin receptors on the follicle, those with a low threshold are preferred). One day, I heard the doctor tell the mother-to-be “you can retrieve your eggs the day after tomorrow” (after the follicle’s diameter and hormone level reach the level of maturity that is considered to be mature at present, and the eggs are retrieved in 36-38h after the HCG injection), and I was very happy. (The eggs are retrieved 36-38 hours after the HCG injection, when the follicle diameter and hormone levels have reached the current level of maturity. Egg retrieval With a strong suction (negative pressure), I entered a long tunnel (egg retrieval needle), and “whoosh”, fell into a huge test tube, I curiously looked at the world around me, dim lights, screen flashing ultrasound machine, and before I had time to see the doctors and nurses in surgical gowns, I was passed into the laboratory, under the microscope, by a glass tube, and was taken to the laboratory, and was then taken to the laboratory. Under the microscope, a glass tube (pasteurized pipette) was picked up into a small plate (Petri dish), brothers and sisters were also successively put in one by one, there is no follicle wall barrier, for the first time, and they had such close contact with each other, shaking each other’s hands, what a novelty of the world. Insemination At 12:00 noon on the same day, we changed to a new home, we were assigned to different small rooms (droplets) in the same big house, and there were new friends (sperms) waiting for us in the small rooms, wow, there were so many of them, densely packed, and the new friends stretched out their friendly hands, and I thought to myself, “Am I going to form a new life with one of them? Who is the most energetic? This is thanks to my good friends around me (granulocytes) and my strong shell (zona pellucida), only through their permission, new friends can come in and form a new life with me. The sperms were scrambling to get through the shell, and finally one got in, at which point my shell changed, and because of the entry of this one sperm, the shell became even harder, and the others were blocked out (known as the zona pellucida reaction, which ensures normal monosperm fertilization, and if more than one sperm enters, then it will either form a multi-prokaryotic embryo or fail to form an embryo, and will be eliminated). Egg splitting Starting at 16:00, I was transferred to another new home (egg splitting dish), and felt a thin, often glass tube blowing, and with the suction of the straw, I tumbled inside the glass tube, and one by one, my good friends (granulosa cells) said goodbye to me (the process of removing the granulosa cells from around the fertilized egg is called egg splitting), and then I and my best friend (granulosa cells) said goodbye to me (the process of stripping the granulosa cells from around the fertilized egg is called egg splitting). The process of removing the granulosa cells from around the fertilized egg is called “egg splitting”), and then my brothers and sisters and I were observed under a microscope by the lab staff to observe the fertilization. Despite the sadness of my friend’s departure, I knew that this was a metamorphosis that I had to go through before becoming a new life. The world around me became clearer and my eyes opened wide to reacquaint myself with this new and strange world. Prokaryotic Score (D1) After a night’s sleep in the warm incubator, I was awakened early in the morning (around 7:30 to 8:00 a.m.), still dim lights, busy people, I re-showered and bathed, and went to the nutrient-rich droplets of the culture, the treatment was good, luxury single room oh (each droplet placed a fertilized egg), I took a big mouthful of the nutrients, I just hope that I can grow up quickly, and return to my mother’s embrace. I just hope I can grow up quickly and return to my mom’s embrace. “2PN” “0PN” “3PN”……, the laboratory staff kept observing and recording. Luckily, I am a “2PN”, and it is said that non-2PN siblings will be eliminated. In the warmth of the incubator, I continued to listen to the outside world: embryo scoring, embryo transfer, embryo re-melting, embryo freezing …… all terms I had never heard of, was this what I was going to experience? Obviously, my body is changing and it feels like my shell is getting more crowded, is it the reason for the higher cell count? Embryo Score (D2) (D3) On the morning of the third day, I moved my body and the coat on my body was noticeably smaller, was I growing again? The same procedure, after being viewed under the microscope, confirmed my feelings, 8 cells, wow! “This embryo is good, evenly divided with relatively little debris, transfer these 2 embryos today”, I don’t know how to express my excitement when I heard this from the teacher who was observing, and I was selected with another good looking embryo. Because of the different growth status, they were discarded either because of too much debris (≥30%) or too slow growth rate (<2 cells/day). Except for the two transferred embryos, the remaining available embryos were either frozen and preserved or cultured in blastocysts, and from then on, my siblings and I were on different sides of the world. Transfer The two lucky ones were transferred to the transfer dish. Under the close cooperation between the laboratory transfer physician and the clinical transfer physician, one of my buddies and I smoothly entered our mother's warm embrace (the uterine cavity), which has a particularly comfortable soft bed. After layers of screening and elimination, lying in my mother's gentle embrace, quietly recalling what happened in the past few days, everything was so new and orderly, the strict checking system, precise operation, and conscientious and responsible work attitude ensured that I grew up healthily, faced the pressure of being eliminated all the time, and finally became the lucky one who could be transplanted, and now I just hope that I can successfully land on the bed, and become a real baby for my mother.