Female: 1, chromosome, blood routine, blood type, liver and kidney function, two-to-one half, hepatitis C, HIV, RPR, TORCH (blood sampling is not related to menstrual cycle); 2, endocrine check on day 2-3 of menstruation: FSH, LH, E2, PRL, P4, TSH, T; 3, white belt routine, cervical cancer smear, mycoplasma, chlamydia, gonococcus; 4, electrocardiogram, breast ultrasound. Male partner: 1, chromosome, blood routine, blood type, liver and kidney function, two-to-half, hepatitis C, HIV, RPR; 2, semen routine, mycoplasma, chlamydia, gonococcus