In vitro fertilization and embryo transfer (IVF-ET), the medical name for IVF, is a method of artificially assisted conception that aims to help infertile couples achieve a healthy child. “IVF also includes a series of derivative techniques: intracytoplasmic sperm injection (ICSI), embryo freezing, embryo assisted hatching (AH), in vitro maturation of immature oocytes (IVM), pre-implantation genetic diagnosis (PGD), pre-implantation genetic screening (PGS), and egg donation. In China, infertile patients can only undergo “IVF” when other methods of pregnancy assistance have failed or are ineffective. Therefore, couples who come to our fertility center need to complete the necessary tests and diagnoses, and are confirmed to be eligible for IVF treatment, such as tubal blockage, ovulation disorders, immune infertility, endometriosis, male oligospermia, obstructive azoospermia, and unexplained infertility, before they can enter the IVF program. “In vitro fertilization – embryo transfer In vitro fertilization-embryo transfer (IVF-ET) is the process of fertilization of sperm and egg in an isolated laboratory environment, followed by the transfer of the formed embryo back to the woman’s uterus. In IVF, the patient first receives medication to develop and mature multiple follicles. When the eggs are about to be expelled, the doctor will insert a puncture needle through the vaginal wall into the mature follicles and aspirate the eggs inside, guided by ultrasound. Analgesics or general anesthesia can help reduce or even eliminate pain during the egg retrieval procedure. In vitro fertilization can be performed in two ways: IVF and ICSI. usually, the embryologist will place the eggs with the preferentially selected sperm and monitor the eggs for fertilization. However, if the male partner’s sperm is of poor quality, or if fertilization in previous IVF cycles was too poor, the embryologist will select one of the most viable and morphologically optimal sperm and inject it into the egg manually to help fertilize the egg, a technique called “intracytoplasmic sperm injection” (ICSI). Pre-implantation genetic diagnosis (PGD) is a diagnostic method used to prevent the birth of children with genetic disorders by performing pre-implantation biopsies and genetic analysis of embryos from patients with genetic risks during in vitro fertilization to select embryos free of genetic disorders for implantation into the uterine cavity. Myotonic dystrophy, fragile X syndrome, nigrostimia, cystic fibrosis, Rh blood group, hemophilia A, sickle cell anemia and thalassemia, progressive dystrophy, neonatal hemolysis, 21 antiprotein deficiency, mucopolysaccharide storage disease, Verhoe’s spinal muscular atrophy, and chromosomal abnormalities such as Down’S syndrome, trisomy 18, Roche The following chromosomal abnormalities can be identified Assisted hatching is the use of physical or chemical methods that are thought to create a defect or gap in the zona pellucida of the embryo to facilitate the emergence of the embryo from the zona pellucida, or to cause the zona pellucida to dissolve and disappear, in order to facilitate embryo hatching and implantation. To increase the likelihood of implantation. Indications for assisted hatching 1. elevated FSH basal level (FSH >15miu/ml on day 3 of menstruation) 2. history of failed IVF treatment, when obvious factors such as endometrium and embryo quality are excluded 3. female partner’s age >38 years old 4. abnormal zona pellucida such as irregular morphology, thick zona pellucida, deep coloration, etc. 5. frozen embryos After a few days, one or more fertilized eggs or embryos will be released into the woman’s uterus through the vagina. This process is called “embryo transfer (ET)”. Transferring the right number of embryos is effective in reducing the incidence of multiple pregnancies. Good quality embryos that are not transferred can be frozen for future use.