The second period after egg retrieval should be ruled out for the following reasons: First, if you have sex, it is recommended to rule out the possibility of pregnancy. Secondly, endocrine disorders leading to menopause should be ruled out. As egg retrieval can cause endocrine disruption, it may lead to menopause, so blood tests for sex hormone 6 and thyroid function should be done to make a preliminary diagnosis. If there is no menstruation after egg retrieval, the possibility of ovarian failure should be ruled out because assisted reproductive technology is used to assist pregnancy, which requires ovulation treatment and artificially removing the eggs and reuniting the sperm and eggs, the ovulation induced in this case may cause the ovarian function to decline.