Many women go to the hospital and after the physical examination and related tests such as mammogram and B-ultrasound, the doctor will recommend the patient to undergo breast puncture biopsy. Many people do not understand how to do the puncture, and some even think that the puncture must be done because they are suffering from breast cancer, so they are very worried and afraid. In fact, such worries are not necessary. Under what circumstances would a specialist recommend a breast puncture biopsy? Generally speaking, when the physician analyzes the physical examination and ancillary examinations and suspects that the breast tumor is suspicious for breast cancer, he/she will recommend the patient to undergo a puncture biopsy. Sometimes, the patient’s breast tumor is not obvious at the time of physical examination, but suspicious lesions are found under ultrasound or mammogram, and DOBI indicates high metabolic or high blood flow signals in the area. A biopsy is more likely. Breast aspiration biopsy is a diagnostic tool that is commonly used: fine needle aspiration biopsy, coarse needle aspiration biopsy, hollow needle aspiration biopsy, and McMurdo tumor excision or biopsy. Different hospitals may use different puncture biopsy methods, but the purpose is the same. Currently, the recommended method is hollow needle or McMurdo biopsy. Puncture biopsy is mostly performed under ultrasound or mammography, which is accurate and reliable, with more biopsy tissues, and is beneficial for pathological diagnosis and immunohistochemical analysis. Surgical excisional biopsy is usually the last biopsy method. It is important to remind every patient that the initial treatment of tumor is very important, therefore, it is also very important to clarify the pathological nature and characteristics of tumor before surgery, which is helpful to help doctors to formulate a scientific and reasonable treatment plan and ensure the successful implementation of treatment. Many people worry that puncture may lead to tumor metastasis, which is not scientifically based. The existing treatment guidelines recommend that it is best to clarify the pathological diagnosis before breast cancer treatment, and in addition, experienced doctors will consider the scope of surgery when choosing the puncture site and remove the puncture site and needle tract as much as possible during surgery, so there is no need to worry. Specialists will choose a reasonable individualized treatment plan according to the patient’s specific situation and under the guidance of treatment guidelines. This is crucial, as different patients have different situations and only individualization can be rationalized, medical science is evolving, and the patient’s age, physical condition, economic status and treatment needs are all elements that doctors have to consider.