In recent years, the incidence of infertility has been increasing year by year due to work pressure, environmental pollution and other factors, troubling many female patients, who are suffering from family pressure and physical and mental blows. Infertility treatment has become the most important and urgent issue. Infertility is not a simple disease, because it is a comprehensive disease caused by more than 150 kinds of reasons, so in order to prescribe the right medicine, it is necessary to find the cause of the disease after a systematic examination, in order to achieve twice the result with half the effort. For the convenience of infertility patients, the routine tests required for infertility are listed, as well as the time and precautions needed for the tests. 1.Endocrine examination: It is recommended to take blood test for sex hormone on the 2nd~5th day of menstruation on an empty stomach, because at this time, the hormone in women’s body will not have too much fluctuation, so it is possible to judge the ovarian function clearly. Those who have irregular menstruation, low menstrual flow and prolonged menstrual period need to do this test. 2.Tubal fluids or imaging test: the test should be done within 3-7 days after menstruation, and avoid sexual intercourse after menstruation. 3.Mycoplasma and Chlamydia test: Mycoplasma and Chlamydia infections can easily lead to pelvic inflammatory disease and tubal blockage, so the test can be done by avoiding menstruation. It is better to avoid sexual intercourse 24 hours before the test. 4, immune examination: immune examination such as anti-sperm antibody, anti-endometrial antibody, anti-ovarian antibody. In recent years, immune infertility accounts for about 30% of infertility. Blood test can be taken, positive antibody will make sperm agglutinate or can lose activity and cause infertility. 5, spousal semen analysis: male factors cause infertility accounts for 20.6% of infertility. Abstinence should be 3~7 days before the examination, and it is better to leave the specimen in the early morning.