Background: Postoperative incisional infection (SSI) after total joint replacement can be devastating for patients, and prevention of SSI remains difficult because of the presence of endografts. Tiffany N. Morrison et al. from Pennsylvania, Philadelphia, suggest that SSI is caused by surgical spread sheets and that re-sterilizing the skin in the operative area prior to applying the incisional protective film may reduce the incidence of SSI. Methods: This study used a randomized, double-blind, prospective approach with a total of 600 patients undergoing total joint replacement from March 2010 to November 2011. The control group used the standard method of skin preparation in the operative area, i.e., preoperative chlorhexidine cleaning, intraoperative alcohol and iodophor disinfection followed by sheeting, and incision protection film after the skin in the operative area had dried. The skin disinfection treatment in the test group was basically the same, but the skin was disinfected once more with Aner’s iodine before applying the incision protection film. There were no differences in other confounding factors between the groups. Results: 581 patients were eventually included in the study. Re-sterilization prior to incisional film application significantly reduced the incidence of SSI in patients with total joint replacement. No SSI occurred in the experimental group (0/284), while the incidence of SSI in the control group was 2.06% (6/297) (p<0.0307). The incidence of skin blistering within 6 weeks after surgery was 3.52% (10/284) in the trial group, which was also lower than that of 6.23% (18/289) in the control group, but there was no difference between the groups (p=0.1745). Discussion and conclusion: Re-sterilization before surgical lamination after sheeting did significantly reduce the incidence of SSI. The authors concluded that re-sterilization kills contaminating pathogens that may be introduced during the sheeting process. Re-sterilization is very meaningful for improving the preparation of the operative area.