The six sex hormone tests are routine tests of the female reproductive system, mainly including testosterone, estradiol, progesterone, luteinizing hormone, prolactin and follicle stimulating hormone: 1) testosterone: normal concentration is 0.7-3.1 nmol/L. If the value of testosterone in the blood is high, consider the possibility of hypertestosteronism or polycystic ovary syndrome; 2) estradiol: the value is 70-1835 pmol/L at the time of ovulation. If the value is low, there is a possibility of ovarian hypofunction, premature ovarian failure, and Silhan’s syndrome; 3, progesterone: 7.6-97.6 nmol/L in late ovulation, if the value is low in late ovulation, consider luteal insufficiency, ovulatory dysfunctional uterine bleeding, etc.; 4, luteinizing hormone: 4-10 mIU/ml in late ovulation, below 5 mIU/ml often indicates gonadotropin insufficiency. Prolactin: during the non-lactation period, the normal value of blood prolactin is 0.08-0.92 nmol/L, higher than 1.0 nmol/L is considered hyperprolactinemia; 6, follicle stimulating hormone: 5-40 mIU/ml is the normal value, low value is seen during estrogen and progestin treatment, Silhan’s syndrome, etc., high value is seen in premature ovarian failure, ovarian insensitivity syndrome, ovarian dysfunction, etc. High values are seen in premature ovarian failure, ovarian insensitivity syndrome, primary amenorrhea, etc. These six tests can give a clear picture of the patient’s endocrine condition and determine whether the ovaries are functioning normally or not, and if the patient has menstrual disorders or endocrine disorders, this test can be done and then treated according to the report. This test is usually performed within the 3rd-5th day after the menstrual period. Before the test, patients should ensure a light diet, get enough sleep and avoid sexual intercourse to avoid affecting the test results. In addition, if necessary, women are recommended to have thyroid function as well as AMH test because thyroid function is closely related to female endocrine function and AMH is a good way to assess ovarian reserve function.