Female: 1, routine gynecological examination (leukorrhea routine, cervical scraping, mycoplasma, chlamydia), anti-sperm antibody examination. 2, blood/urine routine, blood type, coagulation series, liver function, kidney function, infectious disease series, eugenics four, endocrine examination, chromosome examination. 3.Chest X-ray, electrocardiogram, ultrasound, hysterosalpingography. Male side: 1, male physical examination. 2, Routine semen examination (at least 2 times), sperm nuclear staining test, semen leukocyte peroxidase test, anti-sperm antibody test, sperm morphology test. 3, Blood/urine routine, blood type, infectious disease series, chromosome examination.