Bone marrow puncture is less painful and the discomfort is usually painful, which can be tolerated by most of the subjects due to the local anesthetic drugs and can be recovered in about 7 days. However, the degree of pain tolerance and sensitivity varies from person to person and the degree of pain perception varies, and some people may feel more painful. In general, the psychological pressure brought by bone marrow aspiration is also obvious, especially for first-time bone marrow aspiration patients. Excessive nervousness and fear can often increase sensitivity to pain and amplify the painful sensation during the operation. It is recommended that the patient relaxes and does not worry too much as the technique is more mature, and cooperates well with the doctor. Bone marrow puncture usually requires rest for about 7 days after the procedure is completed, and the local bleeding-free wound can usually recover faster after adequate and moderate local pressure after the puncture. However, this operation is an invasive test, and patients need to prevent infection and strictly follow medical advice to care for the wound, pay attention to hygiene, avoid getting water on the wound, and keep it clean and dry. If the wound becomes infected, the healing time will be prolonged accordingly and prompt medical attention is required. Bone marrow aspiration is often used to examine the morphology of blood cells and usually assists in diagnosis, observation of treatment efficacy and prognosis. A skin test is required before injecting anesthetic drugs, and patients with bleeding tendencies should undergo a coagulation test to determine whether they are suitable for such an operation, and bone marrow aspiration is strictly prohibited in patients with hemophilia. The patient can be placed in the supine, lateral, sitting or side-lying position during the examination, depending on the site of the puncture.