The traditional prostate fluid routine examination is flawed in the diagnosis and monitoring of prostatitis. 1. It is difficult to standardize the technique and strength of the massage of the prostate, and there are significant differences in the distribution of leukocytes and lecithin in the prostate fluid from different techniques and strengths. The actual results of the massage only reflect the local situation of the prostate, not the overall condition of the prostate. The results of routine prostate testing are very unstable and poorly reproducible. The results fluctuate greatly when the same patient is examined in different hospitals and at different times within a short period of time, which does not objectively evaluate the changes before and after treatment, so it is difficult to use as a reliable basis for prostate diagnosis and efficacy testing, which also easily leads to doctor-patient conflicts. The advantages of seminal plasma elastase in diagnosing accessory gland infections 1. Semen is mainly composed of the secretions of the prostate and seminal vesicle glands. The ejaculated semen contains the prostatic fluid that is naturally discharged through the prostatic tubules. Replacing the prostatic massage fluid with a semen specimen and quantitatively measuring the elastase content is conducive to the overall evaluation of the infection status of the prostate gland, which, combined with other indicators, can also reflect the infection status of the seminal vesicles, epididymis and other genital tracts. 2. Elastase can be used as a diagnostic and healing test indicator for quiescent genital tract infections, and its reliability has been recognized by the World Health Organization. Some studies have shown that semen elastase concentration correlates well with leukocytes in semen. 3, Clinical practice has proved that the repeatability of elastase test side in semen specimens is better compared with prostate massage fluid, which is especially suitable for monitoring the efficacy after drug treatment. 4, It is generally thought that bacterial infection is the gold standard for diagnosis, but due to the physiological uniqueness of the male accessory gonads, on the one hand prostatic fluid or semen is easily contaminated by the normal flora of the external urethra during sampling, and on the other hand, due to the lack of capacity of existing culture techniques, it is difficult to culture all pathogenic bacteria, so the specificity and sensitivity of prostatic fluid or semen bacterial culture cannot meet the clinical needs. 5. A large number of studies have shown that the concentration of seminal plasma elastase correlates well with the number of leukocytes in the semen. In the case of abnormal seminal plasma elastase concentration, semen leukocyte concentration was >1000ng/ml, but when semen leukocyte concentration was <1< span="">×106/ml, some specimens also had abnormal seminal plasma elastase concentration. Seminal plasma elastase is a sensitive indicator of occult infection in the reproductive tract. 6. Abnormally high seminal plasma elastase concentration is often accompanied by a decrease in the secretory function of the accessory gland (if the sugar content is reduced), as well as a decrease in semen quality (e.g., decreased sperm motility). Antibiotic treatment can effectively reduce the concentration of elastase in semen and improve the symptoms of bacterial prostatitis and sperm quality.