To determine whether it is a fungal infection there are methods such as fungal microscopy, molecular biology identification, fungal culture, etc. The simplest way is fungal microscopy. 1. Fungal microscopy: the simplest and fastest way to directly determine whether it is a fungal infection. However, it is only helpful for subcutaneous and superficial fungal infections, the positive rate is low, and negative results cannot directly exclude the diagnosis. 2. Molecular biology identification: using restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD), fungal DNA alkaline base composition (G+C Mol%), pulsed-field gel electrophoresis (PFGE), Southern blotting rDNA sequencing and other diagnostic methods. 3. Fungal culture: It is an important part of laboratory examination, and the ability to culture pathogenic fungi is a prerequisite for further identification of strains. Routine fungal culture requires 21-28 days of incubation at 28-30°C. Potato agar medium (PDA) or Sartre’s medium (SDA) is usually used for culture. The methods of determining fungal infection include, but are not limited to, the above, with the simplest way being fungal microscopy. However, in case of obvious discomfort, one should immediately go to a specialized hospital for effective treatment.