OBJECTIVE: After perfecting the relevant preoperative evaluation and obtaining the patient’s informed consent, a single-hole multi-channel transabdominal 3D laparoscopic nephrectomy was performed for the first time in China to explore the feasibility and safety of this procedure and to summarize the operational experience. METHODS: On August 5, 2013, a single-hole multi-channel transabdominal 3D laparoscopic nephrectomy (right side) was completed. The patient was diagnosed with “severe hydronephrosis of the right kidney”, so he was given a right nephrectomy. During the operation, a 3-cm-long surgical incision was first made cephalad at 1 cm above the level of the umbilicus at the lateral edge of the right rectus abdominis muscle, and after freeing the renal artery, the renal artery was severed separately, and then the right ureter was severed at the level of the iliac vessels. The kidney was placed in a retrieval bag, the incision was delayed to approximately 4 cm, and removed from the original incision. RESULTS: The procedure was successfully completed without adding any additional incisions, with an operative time of 154 min and intraoperative bleeding of 150 mL. The patient ate on postoperative day 1 after recovery of gastrointestinal function. The postoperative examination of the patient on postoperative day 1 showed that the creatinine was 76 μmol/L. The visual analog pain scores on postoperative days 1, 2, and 3 were 2/10, 1/10, and 0/10, respectively, and the patient was discharged on postoperative day 3 without any intraoperative or postoperative complications without the use of any pain-relieving drugs. CONCLUSION: Single-port multichannel transabdominal 3D laparoscopic nephrectomy is safe, feasible, and effective. Postoperatively, the patient had mild pain, quick recovery, and small incision. Single-hole 3D laparoscopy has good clinical application prospects due to the strong stereoscopic surgical image, high precision of surgical operation, and significantly reduced difficulty of hand-eye coordination.