In vitro fertilization-embryo transfer (IVF-ET), which is commonly referred to as IVF, is an artificial method to fertilize the egg and sperm in vitro, perform early embryo development, and then transfer them to the mother’s uterus for development. Deng Xiaohui of the Fertility Treatment Center of Qilu Hospital of Shandong University The indications for IVF-ET are: 1. Gamete transport disorders caused by various factors in the female partner, such as bilateral tubal obstruction, resection, hydrocele or loss of peristalsis and egg grasping function after tubal inflammation, or adhesions in the pelvic cavity; 2. Ovulation disorders: refractory ovulation disorders that fail to result in pregnancy after repeated conventional treatment or ovulation promotion treatment combined with artificial insemination, such as 3. endometriosis, still not pregnant after surgery or medication; 4. unexplained infertility, all tests are normal and other methods of pregnancy assistance such as artificial insemination have failed many times; 5. immune infertility, ineffective after other methods of treatment; 6. male partner with oligospermia, weak sperm and teratozoospermia, not pregnant after artificial insemination or the severity of the male partner is not suitable for artificial insemination.