The etiology of allergic diseases is complex and diverse, and the causes of sensitization vary from time to time and from place to place. Therefore, the identification of allergens is the primary issue in the diagnosis and treatment of allergic diseases. One of the most classical methods is the application of allergens as stimulants to directly stimulate the patient’s symptoms or the use of allergen avoidance methods, while these tests are complex and usually used in food allergy. For allergy caused by inhaled allergens, the clinical allergy history of the patient is usually combined with an in vivo skin prick test and an in vitro allergen-specific IgE (SIgE) test to determine which allergen the patient inhaled and the extent of the allergy. The skin allergen prick test can confirm or exclude factors that cause allergic disease. The principle is to inject a small amount of active extract of allergens under the epidermis to induce the body to produce IgE, which can bind to IgE receptors on the surface of mast cells in the skin or submucosa, causing the mast cells to degranulate and release large amounts of histamine, slow-reacting substances and other vasoactive substances (allergic mediators), resulting in local vasodilation and exudation, manifesting as a wind mound and erythema. The test result is judged by the diameter of the dermatome. The skin allergen prick test is performed on the skin of the palmar side of the forearm. No special preparation of the skin is required prior to the test. Because any disinfectant and cleaning agent can be an allergen and induce a rash, which can affect the test results. The patient is examined with the arm relaxed and resting on a table. The examiner uses a fine needle tip to gently pierce the skin with a drop of the test solution, allowing a small amount of the test solution to enter the skin. Because the needle only lightly pricks the skin, the patient often feels no pain and the skin does not bleed. The test is often completed unnoticed during a few minutes of relaxed conversation between the doctor and patient, and the results are judged after 20-30 minutes. The examiner uses the diameter of the dermatome as a criterion for judgment. The test is easy to perform, quick and intuitive to observe, painless and easy to accept by the patient, and the price of the test is quite economical. Despite all the advantages, the test does require a few small conditions. Subjects should be as old as possible and older than 5 years old, and patients younger than 5 years old should be tested in small numbers. Discontinue antihistamines, corticosteroids and medications with antihistamine effects for three days prior to the test to avoid false negative results. Do not expose to natural allergens if possible. Testing is prohibited in the following conditions: diseases that significantly impair systemic status, skin lesions at the test site, patients treated with beta blockers or ACE inhibitors, during pregnancy, and during asthma attacks. The causes of allergy are diverse and it is not possible to test the same patient for all allergens at once. Too many choices result in poor compliance. Therefore, there are limitations to the types of allergens selected. We select 30 substances commonly found in the Heze area as allergen testing reagents in a targeted manner, and if necessary, they should be examined in separate tests. The skin allergen prick test not only characterizes the allergen, but also quantifies it so that further desensitization treatment or immune modulation can be performed to change the constitution; if the patient is allergic to food, he/she can refrain from eating the food for the last 2 years.