In vitro fertilization-embryo transfer technique is usually used to remove eggs from female ovaries, fertilize and culture them into embryos with sperm in vitro and then transfer them to the uterine cavity. It is clinically applicable to tubal infertility, endometriosis, ovulation abnormalities and unexplained infertility. 1. Preparation stage: 1. Female side examination items: blood routine, blood type, coagulation function, biochemical function, thyroid function, ovarian reserve function, cervical cancer screening, white belt routine, infectious disease screening, ultrasound, electrocardiogram, chest X-ray, etc.; 2. Male side examination items: blood routine, blood type, biochemical function, semen routine analysis, infectious disease screening, etc. 2. Ovulation promotion stage: use drugs to induce multiple follicles to develop and mature at the same time so as to obtain more high quality eggs and increase the pregnancy rate. Both partners should pay attention to rest, nutrition and hygiene, and intercourse is strictly prohibited during this period. 1. According to different ovulation promotion protocols, the female partner should be injected with ovulation-promoting drugs, and vaginal ultrasound and hormone level check should be performed after the drugs are administered, and the dosage of ovulation-promoting drugs should be adjusted according to the development of follicles. 2. 3. Transfer stage: 1. After HCG injection, the woman will have her eggs retrieved vaginally. There may be pain and discomfort during egg retrieval, and sedative and pain medication will be used to assist. Luteal support is required and blood will be drawn at the transplantation hospital 2 weeks after the procedure to check for pregnancy.