1. Detailed inquiries about the medical history of both parties, including the condition of the fallopian tubes, the condition of the ovaries, the condition of the uterus, etc., as well as previous treatment, to determine the suitability of “test tube fertilization” fertility treatment; 2. To the nurse station to audit the identity card of the two parties, the marriage certificate, the certificate of parental consent, and after passing the test will begin to prepare for the examination; 3. -3 days fasting test female endocrine six, A three; (2) in the menstrual cycle completely clean 2-5 days for hysteroscopy, need to come to the hospital a day in advance to check the leukorrhea, blood, coagulation, electrocardiogram is no abnormality to make an appointment (Qilu Hospital Infertility Clinic Monday to Friday hysteroscopy, holiday exceptions); Shandong University, Qilu Hospital Infertility Clinic Deng Xiaohui (3) fasting check routine blood, urine routine, coagulation series, liver and kidney function, blood glucose, hepatitis B penta, hepatitis C, human immunodeficiency virus (HIV), syphilis antibody, ABO + RH blood type, not subject to the menstrual cycle; (4) vaginal secretions, cervical cytology (TCT) during the non-menstrual period; (5) vaginal ultrasound counting of sinusoidal follicles to learn about ovarian reserve; (6) electrocardiogram, chest X-ray, endometrial biopsy, laparoscopy, immunoantibodies, if needed (6) ECG, laparoscopy, immune antibody examination, etc. 4. Male examination: (1) semen analysis (more than two checklists within one year), autoantibody test and semen microbiology; (2) blood routine, urine routine, blood type, liver and kidney function, blood glucose, Hepatitis B five, Hepatitis C, Human Immunodeficiency Virus (HIV), syphilis antibody (3) semen mycoplasma, chlamydia, gonococcus test (4) to determine the reproductive ability of the man, and to exclude the pathogenicity of the man. (4) Define the male partner’s reproductive ability, exclude organic pathology, and routinely perform external genital examination (5) Introduce the treatment process, costs, and possible risks and complications to both spouses, and sign the informed consent form for the surgery, the legalization of the identity of both spouses, and the informed consent form for the reduction of multiple pregnancies.