Second generation IVF, or second generation IVF, usually selects relatively high quality sperm from the crowd of sperm under the microscope through methods such as gradient centrifugation and completes fertilization through single sperm injection, but it is not possible to pick out the best sperm. Second-generation IVF can effectively reduce the deficiencies in the sperm fertilization process, screen out most of the relatively poor sperm, and select high-quality sperm with good motility and good morphology, thus increasing the success rate of fertilization. In addition, although second generation IVF can select relatively high quality sperm for fertilization, it is difficult to complete the screening if the patient’s own sperm quality is too poor. For patients who have the need for IVF and whose sperm quality is too poor, they can follow the doctor’s instructions to improve their sperm quality by paying attention to rest, abstaining from smoking and drinking, and exercising appropriately before undergoing second-generation IVF. For patients with obstructive azoospermia, testicular or epididymal puncture can be used to obtain sperm and fertilization.