I. Indications for in vitro fertilization and embryo transfer 1. tubal infertility Tubal infertility accounts for about 65% of female infertility. The main factors are bilateral tubal obstruction, hydrocele, severe pelvic adhesions, history of tubal surgery or after resection or ligation. 2. Male factor infertility Male factors such as few, weak, deformed sperm or compound factors leading to male infertility, intrauterine insemination after sperm washing, or patients who fail to obtain pregnancy even after combined use of superovulation-promoting drugs. However, patients with too little sperm or azoospermia due to vasal obstruction may need intracytoplasmic single sperm microinjection technique to help conception. In severe endometriosis, the number and quality of eggs may be affected by the erosion of the ectopic lesions, and the pregnancy rate may be affected. In addition, during the IVF process, the patient may be found to have intrinsic defects of the gametes or fertilization disorders, which may be manifested as non-fertilization or repeated low fertilization rates. fertilization rates, morphologically low quality eggs or abnormal embryo development such as slowed oogenesis and excessive embryo fragmentation. Therefore, IVF as a treatment is also diagnostic for some patients. 5. Persistent polycystic ovary syndrome (PCOS), after repeated (more than 3 times) ovulation-promoting drugs, especially in patients who are insensitive to clomiphene, change to HMG ovulation-promoting to have excess follicular development and prevent the occurrence of OHSS, as well as those who are unsuccessful with repeated ovulation-promoting drugs plus intrauterine insemination, can be changed to IVF-ET for pregnancy. Contraindications to in vitro fertilization and embryo transfer 1. Psychiatric disorders Either of the couple is suffering from serious psychiatric disorders, or is taking anti-schizophrenic or anti-depressant drugs, are not suitable for assisted reproduction techniques. 2. Acute genitourinary infections and sexually transmitted diseases Patients with acute genitourinary infections such as hematuria and proteinuria, acute infection of Chlamydia and gonococcus, active syphilis (positive syphilis reaction, positive RPR) are not suitable for assisted reproductive technology to assist pregnancy. 3.Drug addiction Any one of the couple has serious bad habits such as drug addiction and drug abuse. 4.Radiology, toxic substances, drugs Patients receiving radiological examinations such as chest X-ray, hysterosalpingography, CT, etc. need to wait for 2 months before entering IVF-ET cycle. Exposure to toxic substances such as benzene and lead should not be entered into the cycle. Severe skin diseases such as psoriasis should be entered into the cycle after 3 months of cessation of medication. 5. Severe physical diseases 6. uterine malformation The female partner with severe hypertension, heart disease, abnormal liver and kidney function and kidney disease, who cannot tolerate pregnancy and delivery, should not use assisted reproductive technology to assist pregnancy. If the female partner has double uterine malformation, only one embryo is allowed to be placed, and if it is a twin fetus, reduction of fetus must be performed. No pregnancy function after incomplete hysterectomy.