”In vitro fertilization and embryo transfer (IVF-ET) is a common name for the technique that involves removing an egg from a woman’s body, culturing it in a test tube, adding treated sperm, fertilizing the egg, and continuing to culture it to develop into a few cells that become an early embryo, which is then transferred to the woman’s uterus to develop into a fetus. Since the initial stage of this process takes place in a test tube, it is called “IVF”. It is one of the most widely used assisted reproductive techniques in the treatment of infertility internationally, and most patients with various causes of sexual infertility (infertility) can have their own biological children through this technique. This has become the classic IVF technique. In 1992, Belgian scientists conducting IVF experiments accidentally injected a single sperm into the cytoplasm of an egg and found that the egg so fertilized could also develop into a normal fetus, thus creating the intracytoplasmic single sperm injection (ICSI) technique, which has become an effective treatment for male infertility such as oligospermia, weak sperm and even azoospermia. Some people call it the “second generation IVF” technique, but this is extremely unscientific because it cannot replace the classical IVF technique, but is only a supplement to the classical IVF technique for the treatment of specific infertility conditions, such as oligozoospermia and sperm acrosome dysfunction. With the improvement of embryo manipulation techniques, it is possible to remove a cell for “Pre-implantation Genetic Diagnosis (PGD)” after the zona pellucida (egg shell) is perforated, when the fertilized egg has divided to 6-8 cells, to select a normal embryo for implantation into the uterus, to avoid the birth of babies with genetic defects of parents with genetic diseases, and to achieve eugenics. This technique is also a complement to the classical IVF technique, which is why some people call it the “third generation IVF” technique, which is also extremely unscientific.