Genital herpes is a sexually transmitted disease that is caused by the herpes simplex virus that infects the mucous membrane of the skin in the genital and anal areas of the urinary tract. There are herpes simplex viruses of type 1 and type 2. Herpes virus type 1 is the pathogenic microorganism that causes orofacial herpes, while most genital herpes is caused by the type 2 virus and a small percentage is caused by type 1. The clinical manifestations of genital herpes are diverse, some people will have typical symptoms such as papules, blisters, vesicles and ulcers on the external genitalia, others only show atypical lesions such as non-specific erythema, papules and folliculitis, and some people are asymptomatic virus carriers. 1, antibody test method: commonly used protein blotting method, also available gD2 as antigen detection HSV-2 antibody, with high sensitivity, and can distinguish between HSV-l and HSV-2 advantages. 2, cell culture method: from the bottom of the blister to do tissue culture to isolate the virus, the most sensitive and specific examination method, which takes 5 days – lO days. Because of its high technical requirements and high price, it is not yet commonly used. 3, antigen examination method: such as PCR detection of DNA of skin lesions HSV, high sensitivity and specificity, can greatly improve the ability to confirm the diagnosis of HSV in patients with genital ulcers, but the cost is expensive, and by the operating techniques and laboratory conditions and equipment, prone to false positives, so its accuracy for clinical diagnosis is affected. 4, cytological examination: a small amount of tissue scraped from the bottom of the herpes or ulcer surface for smear, Wright-Giemsa staining or Papanicolaou staining, HSV infection can be detected with the characteristic eosinophilic inclusion bodies in multinucleated giant cells. However, HSV infection or varicella-zoster virus infection cannot be distinguished, and the sensitivity is only 60% of that of virus isolation.