Ozone (O3) is an isomer of oxygen, a molecule with a ring-like structure composed of three oxygen atoms. It is light blue with strong special odor, extremely unstable, and easily decomposed into ○2+○- in air and human tissues. Compared with oxygen, ozone has a large specific gravity, taste, color, soluble in water, easy decomposition, etc., and a half-life of about 20 minutes at room temperature. Ozone is widely used in clinical practice because of its strong oxidizing power and biological effects such as bactericidal, antiviral, anti-inflammatory, analgesic, and immunomodulatory effects, as the ○- atom is very active. Ozone injection is a safe, effective, economical and analgesic treatment with few adverse effects for the treatment of many chronic painful diseases. Ozone can stimulate oxidase overexpression, neutralize reactive oxidation products produced in excess in the inflammatory response, antagonize the release of immune factors in the inflammatory response, dilate blood vessels, improve reflux, and reduce edema around nerve roots. It can also act directly on nerve end-piks and stimulate inhibitory interneurons to release enkephalins and other substances, thus achieving analgesic effects. The indications for ozone treatment of chronic painful diseases: 1, intervertebral disc herniation. 2.Failed Back Surgery Syndrome (FBSS). 3.Soft tissue pain: such as myofascial pain syndrome, third lumbar transverse process syndrome, pear-shaped muscle syndrome, tenosynovitis, tennis elbow, etc. 4.Arthralgia: such as frozen shoulder, knee arthritis, sacroiliac arthritis, hip arthritis, etc. 5, neuropathic pain: such as postherpetic neuralgia, diabetic neuralgia. 6, ischemic pain: such as vasculitis, cerebral ischemia, ischemic necrosis of the femoral head. 7, immune pain: such as rheumatoid, gout, ankylosing spondylitis. 8, cancer pain.