Toxoplasma gondii can be diagnosed clinically by pathogenetic examination and serological examination, in which the pathogenetic examination is more difficult and the positive rate is not high, so serological examination is an important auxiliary diagnostic means widely used at present, that is to say, it is examined mainly by blood sampling.1. Pathogenetic examination: it is mainly examined by smear staining method, some secretion fluid of the patient is centrifuged and then the sediment is taken to make smear, then it is examined by conventional staining method or immunocytochemical method on the smear. Then the smear will be tested by conventional staining method or immunocytochemistry method. Generally, Toxoplasma gondii’s wreaths, chains and clusters can be found in the smear, which can help the diagnosis.2. Serological examination: It is mainly carried out by taking the blood of the patient to be examined and conducting various tests, which can be carried out by staining test, indirect hemagglutination test, indirect immunofluorescence test to detect the antibody against Toxoplasma gondii’s surface membrane in the blood serum, including IgM antibodies and IgG antibodies. If peripheral blood of the mother is drawn before or during early pregnancy and the test is positive for IgM antibodies, it suggests that there may have been a recent infection with Toxoplasma gondii and there is a risk of transmitting the infection to the fetus, which needs to be actively treated; if it is positive for IgG antibodies, it indicates that there was a previous infection with Toxoplasma gondii. If a pregnant woman is tested for toxoplasmosis before pregnancy, it is recommended that she be cured of toxoplasmosis before preparing for pregnancy; if she is already pregnant and found to be suffering from toxoplasmosis, she needs to be actively treated, and if necessary, terminate the pregnancy due to the possibility of fetal malformations.