With the current advances in assisted technology, many patients with severe male infertility are having their own offspring through assisted reproductive technology. 1. What is second-generation IVF? A: Second generation IVF refers to the ICSI technique, which translates to intracytoplasmic single sperm injection in Chinese. It also refers to the process of injecting a sperm into the egg to form an embryo through a microscopic manipulation device. 2. Who is suitable for this technique? A: This technique is mainly suitable for people whose male partner’s sperm quality is particularly poor and who are unable to achieve pregnancy after treatment, including (1) severe oligospermia, weak spermatozoa (sperm concentration <5x10< span="">6/ml, percentage of forward-moving sperm <10%), dead spermatozoa (sperm with no motility, but live sperm confirmed by Eosin staining) (2) obstructive azoospermia (3) non-obstructive (4) Male immune infertility (5) Spermatozoa without acrosome and abnormal acrosome function (6) Severe deformed spermatozoa without genetic reasons (7) Severe retrograde ejaculation 3.Difference between first generation and second generation IVF? A: The two technologies are just different methods of fertilization in the formation of embryos, not that the first generation is better than the second generation or the second generation is better than the first generation, the two technologies are only suitable for patients who need assisted reproduction treatment for different reasons, so if you choose IVF, you don't need to pay too much attention to which technology, doctors will choose according to the situation of both couples.