The first of these was the first of a series of tests to be conducted in the United States. After a thorough physical examination of both men and women, the male partner’s semen routine repeatedly indicated that the sperm count and sperm motility rate were “0”, and after confirmation through puncture surgery and chromosome examination, he was diagnosed with azoospermia. In May, 14 days after the treatment, his wife confirmed that she was pregnant, and they cried tears of joy. With the rapid socio-economic development, the pressure of work and environmental pollution, the number of infertility patients is increasing. According to the survey, about 10-15% of married couples cannot have children, among which male factors account for 20-30%, and azoospermia accounts for about 7-14% of male factors. For azoospermia patients, families with a strong desire to have offspring can achieve this through sperm donation. The diagnosis of male azoospermia needs to be confirmed by a specialist physician at a regular hospital, who will decide on artificial insemination or in vitro fertilization embryo transfer treatment. Due to the tight sourcing of semen, after the diagnosis of azoospermia is confirmed, an appointment must be made in advance and the sperm bank will provide the semen for the corresponding treatment. The state is very strict about the management of sperm donations, and in order to ensure the safe and rational use of semen, the source and destination of each donated sperm must be recorded in detail, and the pregnancy outcome of each sperm must be tracked and followed up. The Reproductive Medicine Center of Nanjing Maternal and Child Health Hospital has received consultations from patients outside and within the province since it passed the audit of artificial insemination by sperm donor technology by the Jiangsu Provincial Health Planning Commission in January 2015. After the standardized examination and strict audit, our center has performed the treatment of donor insemination and donor in vitro fertilization embryo transfer, and two patients have confirmed clinical pregnancy in May. We look forward to serving more azoospermia patients.