1.Basic research SQDB soup can play a synergistic effect on tumor treatment through immune enhancing effect and anti-tumor drugs together. First, SQDB soup has direct inhibitory effects on certain tumors. Ito et al [20] used Ehrlich ascites carcinoma (EAC) and Lewis carcinoma (LLC) mice, and ig or ip SQDB soup inhibited the growth of ascites carcinoma. Hiromu Maruyama et al [8] found that the injection of SQDB soup into the body after resection of cancer inhibited the recurrence of cancer and concluded that the tumor suppression mechanism of this formula can be understood as activation of complement C3, production of tumor necrosis factor (TNF), activation of the reticuloendothelial system and inhibition of the drug metabolizing enzyme system of the tumor. Further experiments [30] found that SQDB soup was used as a biological response modifier and its effect on the immune response of the tumor host was observed by measuring antibody production capacity, macrophage chemotaxis, phagocytosis and intracellular bactericidal function, as well as anti-infective capacity, and the results showed that SQDB soup enhanced a variety of immune responses (its an impaired immune state in the tumor-bearing animals). It promotes the secretion of IL-6 and TNF-α by mononuclear macrophages in the spleen of rats with tumors [31], powerfully induces the activity of CTL and enhances NK cell activity [32]. Experiments using double soft agar medium with clone-forming cell assay showed that SQDB soup has anticancer activity, but it needs to act for a long time to have significant effect. Secondly, SQDB soup can prolong the survival time of experimental animals when used in combination with chemotherapeutic drugs. The combination of TJ-48 and MMC was found to prolong the survival time of P-388 tumor-bearing mice more significantly than that of MMC alone. After 7 d of continuous oral administration of SQDB soup 2. 0g?(g?d) – 1 followed by ipMMC and DDP (cisplatin) for 14 d, it was found by controlled experiments that SQDB soup increased the LD50 of MMC and DDP and significantly prolonged the time to death caused by lethal doses of chemotherapeutic agents [20]. Second, it plays a synergistic role with chemotherapeutic agents while reducing their toxic adverse effects. sQDB soup, when combined with CTX, can effectively inhibit the lung metastasis of LLC cancer and restore the significantly reduced platelet-forming cell response in EL-4 leukemia mice and S-180 sarcoma, while CTX causes no reduction in tumor shrinkage [20]. 50% and 100% concentrations of SQDB soup can significantly increase the LD50 of serum of mice injected with levels of hemolysin in the serum of CTX-injected mice, and had a significant protective and repairing effect on CTX-induced spleen damage [37]. The experiments of Fan Xianghong and Zhou Yonglv et al [12] [15] also demonstrated that SQDB pellets had a significant protective effect and stimulated proliferative effect on the suppression of hematopoietic function in CTX-induced mice.SQDB capsules also significantly increased the T-lymphocyte white fraction ratio in immunocompromised mice induced by hydrocortisone acetate [15], and SQDB soup at 50% and 100% concentrations could significantly increase the serum levels of hemolysin in mice injected with hydrocortisone [16]. The mice were inoculated with S-180 sarcoma (1×106 cells) after oral administration of SQDB broth for 2 g?(g?d) – 1 7d and continued to receive SQDB broth for 30d, during which MMC was administered intravenously on d 3, 8 and 11, and MMC was found to be more effective than SQDB broth alone in inhibiting tumor growth. MMC alone inhibited tumor growth significantly, while increasing the survival of mice and promoting recovery from the atrophy of testes, thymus and spleen caused by MMC, preventing adverse effects such as leukopenia, anemia and weight loss [20]. Wang Yan et al [35] used 150 and 300 mg?kg – 1 of SQDB flush with rectal cancer preferred about murfuralidine (100 mg?kg – 1 ) for 7 d. All three doses of SQDB flush significantly improved the tumor suppression rate of murfuralidine against three tumor strains of transplanted tumors S180, Lewis and H22 in mice: for murfuralidine caused leukocyte decline, immune organ atrophy, macrophage It also improved the activity of NK cells. It is suggested that SQDB soup can significantly improve the side effects of chemotherapy drugs and enhance the cellular and humoral immune functions of mice.Sugiyamak [36] gave S180 tumor-bearing mice ip DDP for 9 d with renal and other adverse effects, while administering SQDB soup for 1. 7 g?(g?d)-1 for 12 d. It was found that SQDB soup could antagonize the anti-tumor activity caused by DDP without affecting the anti-tumor activity of DDP. antagonize the nephrotoxicity, bone marrow toxicity, hepatotoxicity and gastrointestinal toxicity caused by DDP. Another experimenter [37] took human peripheral blood lymphocytes for in vitro test and found that SQDB soup could significantly inhibit the micronucleus experiment induced by CTX, and inferred that SQDB soup and other qi-supplementing and blood-supporting prescriptions were likely to play a protective role against DNA damage. 2. Clinical studies From clinical observations, SQDB soup can significantly improve the cachexia state of cancer patients and enhance the activity of certain immune cytokines through its effect of benefiting qi and nourishing blood and supporting the righteousness to dispel evil. Qi Cong et al [38] randomly divided clinical ovarian, uterine body, and cervical cancer patients into SQDB granules non-dosing group (i.e., group A) and SQDB granules-dosing group (i.e., group B), from 2 weeks before the start of chemotherapy (DDP, 5-FU (5-fluorouracil), ADM (adriamycin), CTX-based combination chemotherapy) to 2 weeks after the end of 8 weeks of chemotherapy for a total of 12 weeks of continuous administration of SQDB granules each time 2 The resultant improvement rate on GI reactions was a mean of 1. 65 meals in group B, a significant increase compared to 1. 12 meals in group A; blood WBC by 8 weeks was 3300 ± 900/ mm3 in group A and 4600 ± 1800/ mm3 in group B. There was no significant difference between the two groups for RBC, Hb, and Ht. In another study [33], 11 patients with brain tumors, who were stabilized after initial treatment with surgery, radiotherapy and chemotherapy, were given continuous oral SQDB soup (7. 5 g?d -1) as maintenance therapy, and after 1 month of administration, suppressor T-cell values decreased in 10 patients, corroborating that SQDB soup can lead to an increase in adjuvant T-cell activity.