A, the experimental examination objects and specific content: 1, who need to do prostate fluid bacterial culture? Prostate massage fluid leukocytes more (++~+++~++++~ leukocytes pus cells full field of view), infertility, urinary disorders, urinary irritation symptoms (urinary frequency and urgency must first do urine culture), perineum and other local pain symptoms! 2, first rule out urinary tract infection, urinary routine, urine culture. 3, strictly prostate fluid to take specimens for general bacterial culture plus drug sensitivity, if the bacterial culture results positive, it is recommended to repeat the general bacterial culture again, especially for the discovery of Staphylococcus epidermidis, E. coli, etc.! 4, if sterile then the next step to do a more rigorous bacterial culture of bacteriophage. 5, it is recommended that the initial culture be performed simultaneously with Mycoplasma solium humanum culture and Chlamydia trachomatis check antigen. 6, a history of purulent discharge from the urethra is recommended for special bacterial smear and gonococcal culture. The actual preparation method: It is best to take oral amoxicillin and other antibiotics that cannot penetrate the prostate for 3 to 4 days before stopping the medication for 5 to 7 days: 1. Prepare disinfected clean underwear, preferably with salt and iodine volts boiled and exposed to the sun! 2, wash the head of the penis before repeatedly washing your hands 3~4 times with a new soap that has never been used. 3, prepare water with salt in an electric kettle to boil, cool and warm, repeatedly wash the head of the penis 4~5 times with new exposure soap, especially the urethral opening, after finishing put on the above prepared underwear to the hospital to take specimens, after washing the head of the penis please never touch the head of the penis urethral opening again. 4, glandular duct blockage prostate fluid can not be eliminated, you can repeatedly aseptic conditions (the specific method as above) to take semen or massage after urine, take semen patients must wear sterile gloves, sterile culture specimen cup can not be exposed to the air again open for too long, more can not take semen in the toilet to do culture but in at least with ultraviolet disinfection conditions of the special laboratory to take semen. 5, doctors must follow the principles of hand hygiene strict operating procedures, must wear a sterile mask hat. Third, such as the case: a patient complained of back and knee pain, frequent urination, urinary urgency, urethra always burning, the course of the disease four years, the abdomen sometimes swelling pain. We asked him to: first do general bacterial culture of the urethra plus drug sensitivity as well as mycoplasma culture of urethral secretions, chlamydia check antigen, to rule out microbial infection of the urethra, then do general bacterial culture of prostate fluid plus drug sensitivity, if ‘sterile’, then do bacteriophage bacterial culture of prostate fluid plus drug sensitivity.