Autologous fat free transplantation has a history of 50 years, with the development of fat suction technology, autologous fat injection has become more and more popular, which has the following advantages: 1, the grafts are autologous tissues, which will not produce immune reaction and rejection reaction. 2, will not cause changes in the body’s endocrine environment, the mammary glands themselves will not be harmed, the future fertility, lactation, there is no adverse effect. 3.Fat particle transplantation is easy to take material, rich source of tissue. 4, fat extraction method is simple, less traumatic, fewer complications, liposuction surgery to take the incision is generally about 3-5mm, so the healing scar is very small and is located in a hidden part. For people with more localized fat accumulation, it can also play a role in weight loss and slimming, and shaping beautiful curves. Especially in recent years, with several research groups have used similar separation methods to separate fat stem cells from adipose tissue, respectively, fat injection can be part of the stem cells injected into the treatment at the same time, so that the fat injection has been more widely used, but the traditional fat injection, due to the fat particles coarse, easy to form nodules, so that its function of injecting into the deep tissues, can’t be injected too superficially, which restricts the application of its in the Skin rejuvenation, and fat stem cell treatment, due to stem cell extraction, long culture cycle, and laboratory-cultured stem cells can not yet be applied to the clinical and other reasons, also limits the use of fat stem cells. We are now using a mechanical way to process the fat, the fat will be made into a suspension, this suspension by immunohistochemical staining confirmed that most of the adipocytes are destroyed, almost no viable adipocytes exist, and cell culture confirmed that the remaining cells are all viable adipose stem cells, so it will be adipose stem cell suspension, so that adipose stem cell injections become possible. The particles are small enough to be injected under the epidermis with a fine needle without nodules, and they have been used in intradermal injections for tear troughs, wrinkles, and dark eye circles, with good results and no complications. The mechanism may be that fat stem cells can stimulate the skin to increase the content of collagen fibers and elastic fibers, increase the elasticity of the skin, improve the quality of the skin, so as to achieve the effect of skin rejuvenation.