Nucleic acid is the general term for DNA and RNA, which can be understood as genes. Nucleic acid testing refers to the genetic sequencing of the patient’s biological specimen to finally determine whether there is a viral infection; the methods of nucleic acid testing are mainly divided into collection of patient specimens and laboratory testing: I. Collection of specimens 1. Nasal and pharyngeal swab sampling: Nasal and pharyngeal swab sampling refers to the extraction of the patient’s nasal or pharyngeal secretions in order to continue the subsequent testing; 2 hours before sampling it is recommended that patients should avoid eating and drinking, and 30 minutes before It is recommended that patients should avoid eating and drinking, smoking, etc. for 30 minutes before sampling to avoid stimulating the nasal or pharyngeal cavity during testing to cause nausea, vomiting and other discomfort; 2. Blood test: the peripheral blood is taken from the patient to detect the level of antibodies in the patient’s blood. A method. Second, laboratory testing 1, reverse transcription-PCR: real-time fluorescence quantitative PCR is the current official use of SARS-CoV-2 confirmation method, which can determine whether the patient specimen contains SARS-CoV-2 and reduce the possible contamination and false positives in the detection process, with the advantages of easy and fast operation, high specificity and high sensitivity; 2, isothermal amplification: isothermal amplification technology detection Cost is relatively low, the equipment required is relatively simple, can achieve rapid detection of pathogens on site, greatly improving detection efficiency; 3, gene chip: gene chip belongs to a new type of detection analysis means, with accurate, rapid characteristics, compared with the traditional pathogen culture method and influenza clinical mimicry, has a higher rate of compliance, specificity and negative predictive value. Third, nucleic acid testing precautions before nucleic acid testing in order to avoid the gathering of people, to reduce the risk of infection, patients can make an appointment online; nucleic acid testing needs to do a good job of personal protection, wear a good mask, and keep a distance of more than one meter from other people; nucleic acid testing should leave the testing site in a timely manner, to avoid staying caused by the gathering of people.