The female snail is the legendary originator of human life creation, she not only tuan earth to create people, but also put themselves as the first matchmaker, respectively, the man and the woman paired into pairs, and teach them to “create people” method, so people have the reproductive function and “self-reliance”, to pass on the seed to the next generation, reproduce! The myths and legends stop here. Myths and legends only stop here, with the development of science, mankind not only revealed the laws of conception of life, but also studied a new way of conception – a sperm plus an egg, placed in a test tube culture, and then implanted in the mother to grow a baby. Many people are curious about how such test-tube babies are actually conceived. What is the unusual process? I take you into the mysterious IVF base – Wuhan University People’s Hospital, Center for Reproductive Medicine, Assisted Reproduction Laboratory. Mysterious “artificial uterus” April 11, the Center for Reproductive Medicine, Assisted Reproduction Laboratory will carry out three “IVF” surgery, including two cases of “first-generation IVF surgery”, one case of “second-generation IVF surgery”, and one case of “second-generation IVF surgery”, and one case of “second-generation IVF surgery”. On April 11th, the Assisted Reproduction Laboratory of the Center for Reproductive Medicine will perform three IVF surgeries, two of which are first-generation IVF surgeries. At 9:00 a.m., the author put on isolation shoes, change into isolation clothing, and then put on a mask, isolation cap, after a change of clothes, into the Center for Reproductive Medicine’s incubation room, that is, the mysterious “artificial uterus”. The air-conditioning-controlled constant-temperature closed culture room is a fully sealed laboratory, no windows, the use of laminar flow facilities on the ceiling to purify the air, a number of computers connected to the LCD, electronic microscopes, large incubators are running quietly, creating a warm and quiet environment. Professor Yang Jing, director of the Center for Reproductive Medicine, said that because the eggs are very demanding on the external environment, in addition to the temperature should be around 37 ℃, humidity and PH value should also be constant, and can not see the bright light, so the laboratory only open two small light is very dark, and only allows up to two people to enter every day. The two ends of the laboratory are connected to the egg collection room and sperm collection room. Eggs and sperm taken from the couple’s bodies are put into test tubes and passed into the lab through a tiny sterilized window. Egg collection is completed within five minutes At this time, Ms. Zhang (a pseudonym), who is about to undergo egg collection surgery, is lying quietly in the operating room. Prof. Yang Jing introduced that Ms. Zhang was infertile due to her husband’s obstructive azoospermia, and arranged the “second generation IVF surgery” for them. After passing the medical examination, Ms. Zhang went to the hospital every day from the third day of her menstrual period to take injections, and the doctor monitored the follicles through ultrasound and blood hormone levels. On the night when the eggs were about to mature, Ms. Zhang was then injected with a special drug to encourage the follicles to finally mature. This was the day Ms. Zhang had her eggs retrieved. Professor Xu Wangming of the Center for Reproductive Medicine, under ultrasound monitoring, held a probe deep inside her body. At that moment, a lotus root hole appeared on the screen, which was an egg cell. Prof. Xu Wangming maneuvered the puncture needle and suctioned each of the “root holes” in turn, sucking out all the fluid inside the follicle, which was yellowish in color, and then followed the puncture needle’s tube into the test tube. Once the follicular fluid is filled, it is passed through a thermostat window and immediately sent to Associate Prof. Long Wen, who is waiting in the lab. Associate Professor Long Wen quickly picked out the eggs under a microscope and injected them into a petri dish of culture fluid that simulated the in vivo environment. In just five minutes, Prof. Xu Wangming had collected 11 eggs for Ms. Zhang. Next, Associate Professor Long Wen removed a small amount of normal sperm from Ms. Zhang’s husband’s testicles by needling. At 2 p.m. on April 11, under an inverted microscope, Associate Professor Long Wen sucked the egg with a thick syringe, and then injected the spermatozoa into the egg by stabbing the center of the spermatozoa with a thin syringe containing the spermatozoa. After the injection, Associate Professor Long Wen will have been injected into the spermatozoa placed in a petri dish, and then into the human uterus humidity, temperature consistent incubator. 11 eggs intracytoplasmic single spermatozoa microinjection is completed, the entire process of less than 4 minutes. Associate Professor Long Wen told reporters that the shorter the time spent outside the incubator, the higher the success rate of pregnancy, so the speed must be fast. The incubator fully simulates the internal environment of the human body, and the temperature is kept at 37℃ all year round. In addition, humidity, carbon dioxide and oxygen concentration, etc. are regulated with reference to the relevant indices of the human body. I noticed that each small glass dish containing sperm eggs are labeled with the names of both parents to avoid confusion caused by the renaming of one party. That morning, also carried out two cases of the first generation of IVF surgery, that is, so that the sperm and egg free combination, not like the last case of the need for artificial “arranged” formation of embryos. The reporter saw through the microscope, the petri dish, many sperm dragged “long tail”, fast swimming; egg is a round sphere, quietly stay, quietly waiting for the sperm to come. These selected sperm will be PK, the final winner will be and egg “cave”, to start their wonderful fertilization process. Prof. Yang Jing introduced that the normally fertilized egg will be put into the new culture solution and continue to be cultured for 24~48 hours. The fertilized egg will divide into 2 cells, 4 cells, 8 cells. When it divides to 8 cells, it can be moved to the woman’s body. Usually this process takes three days. Frozen embryos can be stored permanently Yang Jing, Center for Reproductive Medicine, Hubei Provincial People’s Hospital In the second lab buffer room, there are ten large white jars. Inside the large tanks is water-like liquid nitrogen, quietly keeping the embryos at a low temperature of -196 degrees Celsius, freezing many embryos. Usually, the number of embryos to be transferred is determined by the patient’s age, previous pregnancies and the quality of the embryos. The remaining better embryos are then frozen for preservation. “Frozen embryo transfer is a procedure whereby the embryos or extra embryos at the time of IVF are “slow frozen” in liquid nitrogen at -196 degrees Celsius, and then “quick thawed” when needed, and then implanted into the mother for growth. The embryos are then implanted into the mother’s body for growth. This technique not only improves the success rate of IVF, but also reduces the high cost of repeat IVF. Frozen embryos are usually kept for about two years. “Of course, many couples want to keep them for a longer period of time, and it is possible to keep them permanently after paying the preservation fee. At present, the success rate of IVF completed through this technique at the Reproductive Center of Wuhan Hospital People’s Hospital has reached about 63 percent. Frozen fetus transplantation costs 2,000 yuan.” Prof. Yang Jing said.