1.Indications: tubal obstruction, ovulation disorder, severe endometriosis, severe oligozoospermia in the male partner, obstructive azoospermia, patients with unexplained multiple failures of artificial insemination. 2.Patients who are visiting the clinic for the first time should register and undergo relevant examinations at the outpatient clinic to clarify indications and contraindications. 3.Patients suffering from infectious diseases, medical and surgical diseases and genetic diseases should be informed at the first visit to facilitate further diagnosis and treatment. 4. Pre-operative preparation for patients determined to be in need of IVF: (1) Pre-operative examination: TORCH, two-to-one half, hepatitis C, HIV, syphilis screening, gonococcus, chlamydia, and liver function, blood routine, blood type, sex six on the female side (fasting, morning examination required), semen routine and sperm function test on the male side (2-7 days after different rooms) (2) Trial transplantation on the female side after gynecological examination (3) Severe (4) Prepare marriage certificate, ID card and fertility certificate (valid for one year, stamped by family planning department) for both parties. 5. 6.Patients who have made an appointment should bring all three certificates and preoperative examinations (including originals and copies) in the morning of the second or third day of menstruation, and both parties should go to the IVF receiving room (room 1) at the same time, with the female partner on an empty stomach. 7. The whole cycle will take about two weeks. The female partner will be on continuous medication and will be tested regularly for follicle development, while the male partner will need to come to the hospital on the 2nd-3rd day of menstruation, the day of egg retrieval and the day of transplantation, please schedule in advance. 8. The cost of each IVF cycle is about 15,000 RMB (may vary depending on the protocol and medication used), please prepare about 6,000 RMB when you enter the cycle, the current clinical pregnancy rate in our center is about 40%. 9. Self-test urine HCG 14 days after embryo transfer and inform the result (Tel-08713638247), positive patients please go to the hospital (can be local hospital) for further testing of blood HCG and progesterone value, and continue to keep the fetus. Vaginal ultrasound will be performed 30 days after the transfer to clarify the embryo development, and please feel free to consult if there is bleeding and abdominal pain. Keep the fetus until 8-10 weeks. 10.If the test is negative, stop the luteal support medication, menstruation will come naturally within a week, and patients with frozen embryos can bring all three certificates and checklist to the clinic on the 10th day of the second menstruation for frozen embryo transfer, which takes about one week and costs about 3000 RMB. 11.If you don’t have frozen embryo, you need to bring all three certificates and checklist to the clinic on the 2nd-3rd day of the 4th menstrual period for a new cycle of treatment.