Couples finally undergo IVF after years of tests, treatments, surgeries and long waits. Many people cannot face the reality of not having a child because they always have hope that the next procedure will be successful. Therefore, IVF is the last treatment of choice for patients with infertility. There are high expectations of IVF after repeated failed treatments and the belief that it is the last chance to treat a pregnancy. Women undergoing IVF treatment are highly stressed, the process of IVF treatment is complex, with close doctor-patient collaboration and repeated tests and treatments that can cause emotional changes at any time. Over-emotional stress, excessive physical fatigue, and the stress of excessive financial burden at any stage of the treatment process can lead to IVF failure. Therefore, when pregnancy does not occur (the outcome for most patients) it often makes couples especially vulnerable to anxiety, depression and hostility when they are truly confronted with the fact of infertility for the first time. Patients who undergo IVF-ET treatment, on the one hand, are eager to have a child, and on the other hand, during the treatment, due to hyperovulation and transfer of multiple embryos, they often cause complications such as ovarian hyperstimulation syndrome, multiple pregnancies, ectopic pregnancies, which bring a lot of anxiety to the patients; during the pregnancy, due to the special situation of IVF, pregnant women, after suffering great physical and mental pain, will regard the fetus in their womb as their life They are worried about the physical, intellectual, and developmental problems of the fetus, and the safety of the fetus, so they focus all their attention and energy on the fetus in the womb and no longer engage in social work, and their anxiety is not relieved. For some women, IVF-ET is invasive and painful. During the whole process, patients are prone to fear because they lack the necessary knowledge and understanding of this pregnancy assistance technology. In male percutaneous epididymal sperm retrieval, infertile couples are not only under pressure from society and family, but also unfamiliar with assisted reproductive technology, and are prone to anxiety, restlessness, and doubt; in addition, the epididymis and testicles are hidden and pain-sensitive parts of the male body, which further deepens the fear and anxiety of patients.