In vitro fertilization (IVF) is a technique in which gametes (sperm and eggs) are removed from the human body, fertilized under in vitro conditions to form a fertilized egg and develop into an embryo, and then the resulting embryo is transferred to the uterus for implantation to obtain a pregnancy. There are three types of IVF: 1. In vitro fertilization-embryo transfer (IVF-ET) Indications: gamete transport disorders caused by various factors in the female partner, such as tubal obstruction, absence and post-tubal sterilization; ovulation disorders, such as polycystic ovary syndrome, follicular luteinization unruptured syndrome; endometrial displacement; oligospermia in the male partner, who still cannot get pregnant after general treatment; unexplained infertility, immune infertility. 2. 2nd generation IVF: intracytoplasmic sperm injection (ICSI) Indications: severe oligospermia and malformed spermatozoa in male, obstructive azoospermia, spermatogenic dysfunction (excluding genetic defects), in vitro fertilization failure, immune infertility, sperm acrosome abnormalities, and patients who need preimplantation embryo genetic examination 3. 3rd generation IVF: pre-implantation genetic diagnosis (PGD). It is a high, new and advanced prenatal diagnosis technology developed by combining assisted reproduction technology and molecular biology. Indications: Diagnosis of certain patients with genetic disorders, such as patients with Y chromosome microdeletions and thalassemia. Its advantages include: the ability to diagnose genetic diseases before the embryo is born, eliminating the mental and physical pain caused by maternal induction of labor or the birth of an abnormal baby after prenatal diagnosis. Embryonic chromosomal abnormalities are also a major cause of IVF or ICSI failure in advanced women. The combination of PGD in the treatment of advanced women can reduce age-related chromosomal abnormalities in chromosome related embryos for transfer and improve the success rate of IVF or ICSI. The advent of PGD technology to provide an effective means for eugenics has also given hope for gene therapy techniques. Contraindications to IVF: serious mental illness, acute infection of the genitourinary system, sexually transmitted diseases in either of the couple; diseases that are not easily reproducible under the Maternal and Infant Health Care Act and that are currently not amenable to preimplantation genetic diagnosis or prenatal diagnosis; serious drug addiction or exposure to teratogenic rays, poisons, drugs and in the action period in either of the couple; serious malformation of the uterus in the female partner or suffering from serious physical diseases that do not have the function of pregnancy. Finally, I would like to tell you about the IVF process: the general process is as follows First month: preoperative examination for both men and women. The male partner will go to the hospital in the morning on an empty stomach for 2-7 days, and generally all the preoperative tests can be done in the morning for the male partner, and all the test results can be taken out after a week (except for chromosomes, which takes about a month) for a follow-up with the male doctor. For the female side, the female doctor is the best doctor to see (the second day of menstruation is the best day), the tests are relatively troublesome, once during menstruation and once after menstruation. The documents required to perform IVF can be asked in detail by the nurse at the consultation desk on the first floor while waiting for the test results: valid marriage certificate, ID card and birth certificate issued by the family planning department (there is a template) for both parties, and the birth certificate must be issued for the second child; the name, date of birth and ID number on all documents must be the same. The second month: After the female side is examined without problems, the second month of menstruation two days to come to the clinic, the female doctor according to the specific circumstances of each person will give drugs will be adjusted to promote ovulation, about the end of the second month, the male and female doctors ordered to build medical records (remember to bring all the male side of the previous laboratory tests, including the laboratory tests outside the hospital, artificial insemination required for various documents). The third month: Around the middle of the third month, the female partner retrieves eggs, the male partner retrieves sperm, and transplants after in vitro fertilization.