The clinical significance of the sex hormone test is to understand female endocrine function and diagnose diseases related to endocrine disorders by measuring sex hormone levels. The six sex hormone tests, namely folliculogenic hormone (FSH), luteinizing hormone (LH), estradiol (E2), progesterone (P), testosterone (T), and prolactin (PRL), basically satisfy clinicians’ screening for endocrine disorders and general understanding of physiological functions. The indicators of female hormone levels can change at different ages and periods of the menstrual cycle. Therefore, when interpreting the test results, attention should be paid to the method of testing, the time of the test, the age of the person being tested, the units of the test results (the units of the test results may be different due to different methods of testing, and the range of reference values may also be different), etc., and combined with clinical manifestations (if necessary, other tests should also be referred to) A comprehensive analysis should be performed. The main function of follicle stimulating hormone (FSH) is the development and maturation of follicles in the ovaries. The concentration of FSH is 1.5-10 mIU/ml in the preovulatory period, 8-20 mIU/ml in the ovulatory period and 2-10 mIU/ml in the late ovulatory period. Increase can be seen in premature ovarian failure, ovarian insensitivity syndrome, primary amenorrhea, etc. 2. Luteinizing hormone (LH) is mainly used to promote ovulation and the formation of luteinizing hormone. The concentration of LH is 2-15 mIU/ml in the preovulatory period, 20-100 mIU/ml in the ovulatory period and 4-10 mIU/ml in the late ovulatory period. 5 mIU/ml or less is a reliable indication of low gonadotropin function, which is seen in Silhan syndrome. LH/FSH>=3 is one of the bases for the diagnosis of polycystic ovary syndrome. 3. The main function of prolactin (PRL) is to promote the proliferation of breast milk production and lactation. During the non-lactation period, the normal value of PRL is 102-496uIU/ml. increase is seen during pregnancy and postpartum lactation, anorexia nervosa. 4. The main function of estradiol (E2) is to make the endometrial glands grow into a proliferative phase and promote the development of female secondary sexual characteristics. The concentration of E2 ranges from 13.1-141.96 pg/ml during ovulation, 100.82-500 pg/ml during ovulation, and 74.1-216.1 pg/ml during late ovulation, and decreases in ovarian hypofunction, premature ovarian failure, and Sheehan’s syndrome. The main function of progesterone (P) is to induce the endometrium to change from the proliferative phase to the secretory phase. The blood P concentration is 0-4.8 mnol/L in the pre-ovulatory phase and 7.6-97.6 nmol/L in the post-ovulatory phase. low P value in the post-ovulatory phase is seen in luteal insufficiency and ovulatory uterine dysfunctional bleeding. 6. The main function of testosterone (T) is to promote the development of the clitoris, labia and mons pubis. It has an antagonistic effect on androgens and has a certain influence on systemic metabolism. The plasma testosterone level in women is 2.5-7.3ng/ml, and high T value can cause female infertility.