Sample collection for nucleic acid sample testing through nasal swabs and pharyngeal swabs both have some discomfort, but are usually tolerated by the sampled individual. The nasal swab may be less irritating to the mucosa and cause less discomfort if performed in a standardized manner, but it is less acceptable to the public because it is collected through the nasal cavity. Each person’s tolerance level for discomfort is different and varies to some extent, and can be performed according to individual needs and the requirements of the testing unit. The pharyngeal swab collection process may usually lead to reactions such as coughing, nausea, or even vomiting due to irritation of the pharyngeal mucosa of the sampled person, but it can be recovered relatively quickly after a short period of relief and there are no other problems. Nasal swabs are more difficult to collect than pharyngeal swabs, and health care workers cannot see the collection site accurately, so there may be a certain risk of bleeding, but the health care workers who perform the collection are usually experienced, and the person being collected need not worry excessively. If the subject has chronic pharyngitis or a special structure of the nasal cavity, he or she should promptly communicate with the health care provider to choose the most appropriate test method to avoid greater discomfort and aggravation of the disease or injury. In addition, the mind should be relaxed before the test and fully trust the medical staff, excessive tension may increase the discomfort and also bring obstacles to the collection work. If you are worried that you may vomit after the collection, you can prepare the appropriate items such as plastic bags and tissues in advance, just in case.