Frequently asked questions about tumor drug sensitivity testing: 1. What is tumor drug sensitivity testing? Tumor drug sensitivity testing is an in vitro screening method for chemotherapeutic drugs that are sensitive to tumor patients. It can assist and guide clinicians to select sensitive chemotherapeutic drugs for each patient to achieve case-by-case treatment. 2.What are the advantages of ATP-biofluorescence in vitro tumor drug sensitivity test compared with traditional in vitro tumor drug sensitivity test? ATP-Biofluorescence in vitro tumor drug sensitivity assay reflects the killing ability of chemotherapeutic drugs on tumor cells by measuring the content of endogenous ATP in cells. It has high sensitivity, high evaluability, high throughput screening, good stability and reproducibility, computerized scanning and software analysis compared to traditional tumor drug sensitivity assays (e.g. MTT method), and can detect high values even when the number of tumor cells is small, making the in vitro results highly consistent with the in vivo treatment response. Positive and negative predictive values, sensitivity and specificity are higher than other techniques. 3.What patient specimens are required for ATP-biofluorescence in vitro tumor drug sensitivity testing? ATP-biofluorescence in vitro tumor drug sensitivity test requires active patient’s tumor cells, so the specimens to be sent can be the tumor tissues (not less than 1cm3) cut down during surgery, chest and peritoneal fluid (not less than 200ml) with cancer cells detected during treatment, metastatic lymph nodes removed, and bone marrow extracts from hematologic tumors. All of the above specimens are the more the better. During transportation to the laboratory, tumor tissues need to be immersed in sterile saline or culture medium and stored at 4°C; thoracic and ascitic fluid specimens are stored in sterile saline bottles or negative pressure bags with appropriate heparin anticoagulation; bone marrow specimens are stored in anticoagulation tubes. Specimens should be sent to the laboratory as soon as possible and generally should not exceed 24 hours. Note: Specimens sent for examination should not undergo any treatment. For specimens sent for pathological examination (i.e. formaldehyde fixed, paraffin embedded), no further tumor drug sensitivity testing should be performed. Surgical patients have only one chance, so please cherish this opportunity for patients and their families to avoid missing the good opportunity. 4.What chemotherapy drugs can be detected by ATP-Biofluorescence in vitro tumor drug sensitivity test? In principle, various chemotherapeutic drugs and their combinations can be detected. Patients can be tested according to the chemotherapy regimen provided by the doctor or the chemotherapy regimen summarized by the center itself. 5.When will the results of ATP-Biofluorescence in vitro tumor drug sensitivity test be available? The results will be available in 5-7 days.