Occupational gallbladder lesions include gallbladder polyps, gallbladder cancer, gallbladder adenomyosis, gallbladder adenoma, and other related diseases. In order to improve inpatient bed utilization, effectively utilize medical resources, and reduce patient hospitalization costs while maximizing patient convenience, relevant tests in the perioperative period of LC must be quick and effective. Ultrasound is a commonly used imaging technique in the diagnosis and differential diagnosis of gallbladder diseases. Although gallbladder-occupying lesions have certain characteristics on ultrasound images, conventional two-dimensional ultrasound is still very difficult in the differential diagnosis of different pathological types and lesions that are morphologically very close to each other. Ultrasonography, a new ultrasound imaging technique applied to gallbladder-occupying lesions in recent years. In developed countries, laparoscopic cholecystectomy can already be performed in outpatient operating rooms, clinics or separate wards in hospitals, and day surgery specialty wards are increasingly being used for laparoscopic cholecystectomy in China. In order to improve the utilization of inpatient beds, effective use of medical resources and reduce the cost of hospitalization for patients. At the same time it can maximize patient convenience, perioperative related investigations must be quick and effective. Although gallbladder polyp-like lesions have certain characteristics on ultrasound images, they lack specificity, such as broad-based solitary gallbladder polyps, early thick-walled gallbladder carcinoma, limited adenomyomatous hyperplasia of the gallbladder, and biliary sludge deposition disease that does not vary with body position, for which conventional two-dimensional ultrasound still has difficulties in differential diagnosis. Ultrasonography has become a fast and convenient imaging method with accurate and reliable results because it can display the blood flow signal in the lesion in real time. Ultrasound imaging of gallbladder cancer often shows heterogeneous hypoechoic nodules in the wall or lumen of the gallbladder, with rapid hyperenhancement in the arterial phase, less uniform distribution and multiple irregular vascular structures, poor continuity of the gallbladder wall structure where the lesion is attached, and disappearance of normal structures. 2. Ultrasound imaging of gallbladder polyps often shows synchronous isoenhancement with or slightly above the gallbladder wall in the arterial and delayed phases. 3. The ultrasonography of hyperplasia shows a limited thickening of the gallbladder wall, which is seen in the arterial phase with synchronous enhancement of the gallbladder wall in a ring shape, where the gallbladder wall structure is continuous and intact. Ultrasonography has a high accuracy rate for the diagnosis of benign and malignant gallbladder occupying lesions. Cholesterol polyps account for the largest proportion of benign occupying lesions of the gallbladder. The diagnostic accuracy of ultrasonography for cholesterol polyps and adenomatous hyperplasia of the gallbladder was high, but the diagnostic accuracy for adenoma of the gallbladder was low, and it was most often misdiagnosed as cholesterol polyps. In conclusion, ultrasonography has a high diagnostic accuracy for gallbladder space-occupying lesions, which can significantly shorten the waiting time for relevant tests before LC surgery and provide more reliable diagnostic information for clinical practice. However, further studies are needed to distinguish the pathological typing of gallbladder polyps, special types of gallbladder polyps, gallbladder adenoma and gallbladder cancer by ultrasonography.